User:Tara K. Luckau/Notebook/Team ConGen/2012/01/06: Difference between revisions

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* prepping to start multiplexing CNHY primers ... so see primer interactions ...
* prepping to start multiplexing CNHY primers ... so see primer interactions ...
===Gradient Summary===
* green cells indicate clean amplification; orange cells indicate less-than-ideal conditions, but useable if necessary
* [[Image:20120106 GradientSummary.png]]
===Multiplex Map===
* markers placed arbitrarily
* marker ranges indicated as in Crawford et al 2007, but approximate amplicon size may be given in final parentheses if Crawford designation is wide or very different from gel.
* [[Image:20120106 Map.png]]




Revision as of 14:58, 6 January 2012

Tara's Lab Notebook <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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CNHY Multiplex

  • prepping to start multiplexing CNHY primers ... so see primer interactions ...


Gradient Summary

  • green cells indicate clean amplification; orange cells indicate less-than-ideal conditions, but useable if necessary


Multiplex Map

  • markers placed arbitrarily
  • marker ranges indicated as in Crawford et al 2007, but approximate amplicon size may be given in final parentheses if Crawford designation is wide or very different from gel.


AutoDimer

  • color families (red, green, blue, gray) indicate possible multiplexing based on primer interactions



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