User:Tara K. Luckau/Notebook/Team ConGen/2012/01/06

ASHY Multiplex

• prepping to start multiplexing CNHY primers ... so see primer interactions ...

Gradient Summary

• green cells indicate clean amplification; orange cells indicate less-than-ideal conditions, but useable if necessary
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Multiplex Map

• markers placed arbitrarily
• marker ranges indicated as in Crawford et al 2007, but approximate amplicon size may be given in final parentheses if Crawford designation is wide or very different from gel.
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AutoDimer

• color families (red, green, blue, gray) indicate possible multiplexing based on primer interactions
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PCR - determine approximate range of each marker

• pool high-quant DNA samples from throughout SD county:

-- CNHY CPN 02

-- CNHY LOM 08

-- CNHY RAJ 01

-- CNHY RAJ 35

-- CNHY RAJ 42

-- CNHY RAJ 50

-- CNHY TP1 11

-- CNHY TP3 05

-- CNHY TP3 19

-- CNHY TP3 20

• 2µL each DNA STK + 80µL LowTE (to dilute to ~10ng/µL) + 20µL CNHY 062911TKLH ('play' DNA, already 10 ng/µL)