User:Tara K. Luckau/Notebook/Team ConGen/2012/01/14: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
No edit summary
No edit summary
Line 12: Line 12:




===PCR===
===PCR - Uniplexes===
* uniplex to determine marker range
* individual CNHY and CNTI DNAs to determine marker quality when uniplexed
* pooled CNHY and CNTI DNAs to determine marker range when uniplexed
* [[Image:20120114_PCRa.png|800 px]]
* [[Image:20120114_PCRa.png|800 px]]




===PCR===
===PCR - Multiplex all-in-one===
* multiplex to determine general marker quality and strength
* individual CNHY and CNTI DNAs to determine marker quality when multiplexed
* pooled CNHY and CNTI DNAs to determine marker range when multiplexed
* [[Image:20120114_PCRb.png|800 px]]
* [[Image:20120114_PCRb.png|800 px]]




===PCR===
===PCR - Multiplex A and Multiplex B===
* split multiplex into two (no post-PCR mixing) based on primer interactions, gradient results, and estimated amplicon size
* compare all-in-one multiplex with Multiplex A and B - better?
* compare scores in multiplex A and B with uniplex to make sure scores match and nothing weird is happening
* [[Image:20120114_PCRc.png|800 px]]
* [[Image:20120114_PCRc.png|800 px]]


Revision as of 20:29, 14 January 2012

Tara's Lab Notebook <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>


CNHY / CNTI Multiplex

PCR - Uniplexes

  • uniplex to determine marker range
  • individual CNHY and CNTI DNAs to determine marker quality when uniplexed
  • pooled CNHY and CNTI DNAs to determine marker range when uniplexed


PCR - Multiplex all-in-one

  • multiplex to determine general marker quality and strength
  • individual CNHY and CNTI DNAs to determine marker quality when multiplexed
  • pooled CNHY and CNTI DNAs to determine marker range when multiplexed


PCR - Multiplex A and Multiplex B

  • split multiplex into two (no post-PCR mixing) based on primer interactions, gradient results, and estimated amplicon size
  • compare all-in-one multiplex with Multiplex A and B - better?
  • compare scores in multiplex A and B with uniplex to make sure scores match and nothing weird is happening





Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Tara_K._Luckau/Notebook/Team_ConGen/2012/01/14&oldid=576119"