User:Tara K. Luckau/Notebook/Team ConGen/2012/01/16: Difference between revisions

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==CNTI Acos5==
==CNHY / CNTI Multiplex==


===PCR===
* [[Image:20120110_PCRa.png|800 px]]
===Gel===


{| {{table}} style="text-align: center"
{| {{table}} style="text-align: center"
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[[Image:20120110_Gela.png|800 px]]
===Gel - Uniplexes===
 
 
* [[Image:20120116_Gela.png|800 px]]
* conclusions
 
 
===Gel - Multiplex all-in-one===
 
 
* [[Image:20120116_Gelb.png|800 px]]
* conclusions
 
 
===Gel - Multiplex A and Multiplex B===
 
 
* [[Image:20120116_Gelc.png|800 px]]
* conclusions


* mispriming everywhere - primer pair not usable for CNHY
* lots of mispriming, but may be able to use conditions indicated by orange face ([[Image:orangefrownyface.png|20 px]]), if needed, under stringent conditions
* favorable conditions indicated by [[Image:greenhappyface.png|25 px]]




Revision as of 20:32, 14 January 2012

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CNHY / CNTI Multiplex

Pour Load Run
270 mL 1x TAE + 5.4 g agarose 2 µL gel load dye + 4 µL PCR product 160 V
27 µL GelRed 6 µL ladder 45 minutes


Gel - Uniplexes


Gel - Multiplex all-in-one


Gel - Multiplex A and Multiplex B




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