User:Tara K. Luckau/Notebook/Team ConGen/2012/01/19: Difference between revisions

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[[Image:20120119_Gel.png|800 px]]
[[Image:20120119_Gel.png|800 px]]


* conclusions
* in CNHY / CNTI Uniplexes, 2 out of 3 amplified; but previous uniplex amplified well, so we'll go with it
* in SCOC Scun10 trials, good banding, water is clean
 
 
* send to frag!




Revision as of 16:14, 19 January 2012

Tara's Lab Notebook <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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CNHY / CNTI Multiplex

PCR


SCOC Multiplex

PCR


Gel

  • used gel made 17Jan2012


Pour Load Run
270 mL 1x TAE + 5.4 g agarose 2 µL gel load dye + 4 µL PCR product 140 V
27 µL GelRed 6 µL ladder 40 minutes


  • in CNHY / CNTI Uniplexes, 2 out of 3 amplified; but previous uniplex amplified well, so we'll go with it
  • in SCOC Scun10 trials, good banding, water is clean


  • send to frag!




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