User:Tara K. Luckau/Notebook/Team ConGen/2012/01/23: Difference between revisions

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==CNTI Acos5==
==Fragment Analysis Results==


UAGC Code: RRAX
PCRs from 19 January 2012


===PCR===
* [[Image:20120110_PCRa.png|800 px]]


===SCOC Scun10 in PET===
* looks great! keep it here
* [[Image:20120123_Scun10.png|800 px]]


===Gel===


{| {{table}} style="text-align: center"
===ASHY Multiplex===
|-
* clean peaks in 3 remaining markers, for the most part
! Pour !! Load !! Run
|-
| 270 mL 1x TAE + 5.4 g agarose || 2 µL gel load dye + 4 µL PCR product || 160 V
|-
| 27 µL GelRed || 6 µL ladder || 45 minutes
|}




[[Image:20120110_Gela.png|800 px]]
====Acos5 - 150-160====
* monomorphic per species
: CNHY @ 154 bp
: CNTI @ 152 bp
* [[Image:20120123_Acos5.png|800 px]]
 
 
====Ai5035 - 240-290====
* polymorphic
* [[Image:20120123_Ai5035.png|800 px]]
 
 
====Ai5062 - 215-275====
* polymorphic
* [[Image:20120123_Ai5062.png|800 px]]


* mispriming everywhere - primer pair not usable for CNHY
* lots of mispriming, but may be able to use conditions indicated by orange face ([[Image:orangefrownyface.png|20 px]]), if needed, under stringent conditions
* favorable conditions indicated by [[Image:greenhappyface.png|25 px]]




Revision as of 16:37, 15 March 2012

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Fragment Analysis Results

UAGC Code: RRAX PCRs from 19 January 2012


SCOC Scun10 in PET

  • looks great! keep it here


ASHY Multiplex

  • clean peaks in 3 remaining markers, for the most part


Acos5 - 150-160

  • monomorphic per species
CNHY @ 154 bp
CNTI @ 152 bp


Ai5035 - 240-290

  • polymorphic


Ai5062 - 215-275

  • polymorphic




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