User:Tara K. Luckau/Notebook/Team ConGen/2012/03/15: Difference between revisions

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==CNTI Acos5==
==State of the Laboratory Work==




===PCR===
===SCOC Multiplex===
* [[Image:20120110_PCRa.png|800 px]]
* nearly fully optimized for pseudo-multiplex (10 markers, 4 PCRs, 1 frag run)
* Scun10 looks great in PET
* sar80 will need to be run in uniplex
* verify Scun3 and Scun11 marker ranges in small diversity panel




===Gel===
===CNHY Multiplex===
 
*  
{| {{table}} style="text-align: center"
|-
! Pour !! Load !! Run
|-
| 270 mL 1x TAE + 5.4 g agarose || 2 µL gel load dye + 4 µL PCR product || 160 V
|-
| 27 µL GelRed || 6 µL ladder || 45 minutes
|}
 
 
[[Image:20120110_Gela.png|800 px]]
 
* mispriming everywhere - primer pair not usable for CNHY
* lots of mispriming, but may be able to use conditions indicated by orange face ([[Image:orangefrownyface.png|20 px]]), if needed, under stringent conditions
* favorable conditions indicated by [[Image:greenhappyface.png|25 px]]




Revision as of 16:45, 15 March 2012

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State of the Laboratory Work

SCOC Multiplex

  • nearly fully optimized for pseudo-multiplex (10 markers, 4 PCRs, 1 frag run)
  • Scun10 looks great in PET
  • sar80 will need to be run in uniplex
  • verify Scun3 and Scun11 marker ranges in small diversity panel


CNHY Multiplex




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