User:Tara K. Luckau/Notebook/Team ConGen/2012/07/06: Difference between revisions

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==CNTI Acos5==
==Field Work==




===PCR===
* July 3-6: SYR
* [[Image:20120110_PCRa.png|800 px]]




===Gel===
===SYR Tissue Count - this week===
 


{| {{table}} style="text-align: center"
{| {{table}} style="text-align: center"
|-
|-
! Pour !! Load !! Run
! !! A <br /><small>TP3 11-15 <br /> TP4 1-7</small> !! B <br /><small>TP3 1-10</small> !! C <br /><small>TP1 1-10</small>
|-
|-
| 270 mL 1x TAE + 5.4 g agarose || 2 µL gel load dye + 4 µL PCR product || 160 V
| CNTI || 0 || 0 || ?
|-
|-
| 27 µL GelRed || 6 µL ladder || 45 minutes
| SCOC || 0 || 0 || ?
|}
|}




[[Image:20120110_Gela.png|800 px]]
===SYR Tissue Count - New Totals===
 


* mispriming everywhere - primer pair not usable for CNHY
{| {{table}} style="text-align: center"
* lots of mispriming, but may be able to use conditions indicated by orange face ([[Image:orangefrownyface.png|20 px]]), if needed, under stringent conditions
|-
* favorable conditions indicated by [[Image:greenhappyface.png|25 px]]
! !! A <br /><small>8, 32, 33</small> !! B <br /><small>9, 10, 29, 11, 25</small> !! C <br /><small>13, 14</small>
|-
| CNTI || style="background: orange" | 24 || style="background: Chartreuse" | 36 || style="background: red" | 9
|-
| SCOC || style="background: Chartreuse" | 36 || style="background: Chartreuse" | 42 || style="background: orange" | 21
|}




Revision as of 13:39, 5 July 2012

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Field Work

  • July 3-6: SYR


SYR Tissue Count - this week

A
TP3 11-15
TP4 1-7		 B
TP3 1-10		 C
TP1 1-10
CNTI 0 0 ?
SCOC 0 0 ?


SYR Tissue Count - New Totals

A
8, 32, 33		 B
9, 10, 29, 11, 25		 C
13, 14
CNTI 24 36 9
SCOC 36 42 21




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