User:Tara K. Luckau/Notebook/Team ConGen/2012/07/17: Difference between revisions

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===PCR MP1a and MP1b===
===PCR MP1a and MP1b===
* Primer pairs grouped into the two multiplexes based on [http://openwetware.org/wiki/User:Tara_K._Luckau/Notebook/Team_ConGen/2012/01/06 primer interactions] (especially [http://openwetware.org/wiki/User:Tara_K._Luckau/Notebook/Team_ConGen/2012/01/13 interactions of concern]), [http://openwetware.org/wiki/User:Tara_K._Luckau/Notebook/Team_ConGen/2012/01/06 annealing temperature], and [http://openwetware.org/wiki/User:Tara_K._Luckau/Notebook/Team_ConGen/2012/01/23 amplicon size]
* Primer pairs grouped into the two multiplexes based on:
:: - [http://openwetware.org/wiki/User:Tara_K._Luckau/Notebook/Team_ConGen/2012/01/06 primer interactions] (especially [http://openwetware.org/wiki/User:Tara_K._Luckau/Notebook/Team_ConGen/2012/01/13 interactions of concern])
:: - [http://openwetware.org/wiki/User:Tara_K._Luckau/Notebook/Team_ConGen/2012/01/06 annealing temperature]
:: - [http://openwetware.org/wiki/User:Tara_K._Luckau/Notebook/Team_ConGen/2012/01/23 amplicon size]
* all primers at same concentration
* all primers at same concentration
* GOAL: to see whether all loci are amplifying and at what intensity
* GOAL: to see whether all loci are amplifying and at what intensity

Revision as of 16:01, 18 July 2012

Tara's Lab Notebook <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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SCOC MP1

PCR MP1-G+Y

  • try to combine MP1-G with MP1-Y (same buffer and annealing temperature, limited primer interactions)


CNHY/TI MP1

PCR Uniplex Ai5033

  • Uniplex


PCR MP1a and MP1b

  • Primer pairs grouped into the two multiplexes based on:
- primer interactions (especially interactions of concern)
- annealing temperature
- amplicon size
  • all primers at same concentration
  • GOAL: to see whether all loci are amplifying and at what intensity





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