User:Tara K. Luckau/Notebook/Team ConGen/2012/07/18: Difference between revisions

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==SCOC ==
==SCOC MP1, CNHY/TI MP1==




===Gel===
===Gel===


{| {{table}} style="text-align: center"
(used previously-made gel)
|-
! Pour !! Load !! Run
|-
| 270 mL 1x TAE + 5.4 g agarose || 2 µL gel load dye + 4 µL PCR product || 160 V
|-
| 27 µL GelRed || 6 µL ladder || 45 minutes
|}




[[Image:20120110_Gela.png|800 px]]
[[Image:20120718_Gel.png|800 px]]


* mispriming everywhere - primer pair not usable for CNHY
* conclusions here
* lots of mispriming, but may be able to use conditions indicated by orange face ([[Image:orangefrownyface.png|20 px]]), if needed, under stringent conditions
* favorable conditions indicated by [[Image:greenhappyface.png|25 px]]




Revision as of 16:03, 18 July 2012

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SCOC MP1, CNHY/TI MP1

Gel

(used previously-made gel)


  • conclusions here




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