User:Tara K. Luckau/Notebook/Team ConGen/2012/07/27: Difference between revisions

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==CNTI Acos5==
==Field Work==




===PCR===
* July 9-13: TP4
* [[Image:20120110_PCRa.png|800 px]]




===Gel===
===TP Tissue Count - this week===
 


{| {{table}} style="text-align: center"
{| {{table}} style="text-align: center"
|-
|-
! Pour !! Load !! Run
! !! A <br /><small>TP3 11-15 <br /> TP4 1-7</small> !! B <br /><small>TP3 1-10</small> !! C <br /><small>TP1 1-10</small>
|-
|-
| 270 mL 1x TAE + 5.4 g agarose || 2 µL gel load dye + 4 µL PCR product || 160 V
| CNHY || 0 || 0 || 0
|-
|-
| 27 µL GelRed || 6 µL ladder || 45 minutes
| SCOC || 0 || 0 || 0
|}
|}




[[Image:20120110_Gela.png|800 px]]
===TP Tissue Count - New Totals===
 
* mispriming everywhere - primer pair not usable for CNHY
* lots of mispriming, but may be able to use conditions indicated by orange face ([[Image:orangefrownyface.png|20 px]]), if needed, under stringent conditions
* favorable conditions indicated by [[Image:greenhappyface.png|25 px]]




{| {{table}} style="text-align: center"
|-
! !! A <br /><small>TP3 11-15 <br /> TP4 1-7</small> !! B <br /><small>TP3 1-10</small> !! C <br /><small>TP1 1-10</small>
|-
| CNHY || style="background: orange" | 29 || style="background: Chartreuse" | 30 || style="background: orange" | 19
|-
| SCOC || style="background: Chartreuse" | 39 || style="background: Chartreuse" | 40 || style="background: orange" | 19
|}




Revision as of 09:21, 25 July 2012

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Field Work

  • July 9-13: TP4


TP Tissue Count - this week

A
TP3 11-15
TP4 1-7		 B
TP3 1-10		 C
TP1 1-10
CNHY 0 0 0
SCOC 0 0 0


TP Tissue Count - New Totals

A
TP3 11-15
TP4 1-7		 B
TP3 1-10		 C
TP1 1-10
CNHY 29 30 19
SCOC 39 40 19



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