User:Tara K. Luckau/Notebook/Team ConGen/2012/07/31: Difference between revisions

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==CNTI Acos5==
==Fragment Analysis Submission==




===PCR===
* 3µL MP1-B + 3µL MP1-G + 3µL MP1-Y
* [[Image:20120110_PCRa.png|800 px]]




===Gel===
* [[Image:20120731_Frag.png|900 px]]


{| {{table}} style="text-align: center"
|-
! Pour !! Load !! Run
|-
| 270 mL 1x TAE + 5.4 g agarose || 2 µL gel load dye + 4 µL PCR product || 160 V
|-
| 27 µL GelRed || 6 µL ladder || 45 minutes
|}


* FedEx Tracking# [http://www.fedex.com/Tracking?language=english&cntry_code=us&tracknumbers=876927708843 876927708843]
:: [[Image:20120731_FedEx.png|700 px]]


[[Image:20120110_Gela.png|800 px]]


* mispriming everywhere - primer pair not usable for CNHY
* UAGC Submission# VXYC
* lots of mispriming, but may be able to use conditions indicated by orange face ([[Image:orangefrownyface.png|20 px]]), if needed, under stringent conditions
* favorable conditions indicated by [[Image:greenhappyface.png|25 px]]




* FedEx picked up 2:43pm, 19 July
* FedEx delivered 9:21am, 20 July
* UAGC received 11:52am, 20 July
* UAGC completed 9:54am, 23 July




Revision as of 13:56, 31 July 2012

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Fragment Analysis Submission

  • 3µL MP1-B + 3µL MP1-G + 3µL MP1-Y




  • UAGC Submission# VXYC


  • FedEx picked up 2:43pm, 19 July
  • FedEx delivered 9:21am, 20 July
  • UAGC received 11:52am, 20 July
  • UAGC completed 9:54am, 23 July



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