User:Tara K. Luckau/Notebook/Team ConGen/2012/10/19: Difference between revisions

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==CNTI Acos5==
==SCOC Batch 2==
* Samples = USGS SYR & LOM + Clark HOL & RAJ




===PCR===
===PCR===
* [[Image:20120110_PCRa.png|800 px]]
* MP1-G
 
* [[Image:20121019_PCR.png|800 px]]
 
===Gel===
 
{| {{table}} style="text-align: center"
|-
! Pour !! Load !! Run
|-
| 270 mL 1x TAE + 5.4 g agarose || 2 µL gel load dye + 4 µL PCR product || 160 V
|-
| 27 µL GelRed || 6 µL ladder || 45 minutes
|}
 
 
[[Image:20120110_Gela.png|800 px]]
 
* mispriming everywhere - primer pair not usable for CNHY
* lots of mispriming, but may be able to use conditions indicated by orange face ([[Image:orangefrownyface.png|20 px]]), if needed, under stringent conditions
* favorable conditions indicated by [[Image:greenhappyface.png|25 px]]




* Errors:
: Scun9-F-HEX: only had ~7 µL left
: Scun19-R-pig: only had ~46 µL left
: added 50 + 30 = 80 µL H<sub>2</sub>O to make up for volume lost




Revision as of 14:59, 25 October 2012

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SCOC Batch 2

  • Samples = USGS SYR & LOM + Clark HOL & RAJ


PCR

  • MP1-G


  • Errors:
Scun9-F-HEX: only had ~7 µL left
Scun19-R-pig: only had ~46 µL left
added 50 + 30 = 80 µL H2O to make up for volume lost



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