User:Tara K. Luckau/Notebook/Team ConGen/2012/10/19

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(Autocreate 2012/10/19 Entry for User:Tara_K._Luckau/Notebook/Team_ConGen)
Current revision (12:43, 26 October 2012) (view source)
 
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==CNTI Acos5==
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==SCOC Batch02==
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* Samples = USGS SYR & LOM + Clark HOL & RAJ
===PCR===
===PCR===
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* [[Image:20120110_PCRa.png|800 px]]
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* MP1-G
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* [[Image:20121019_PCR.png|800 px]]
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===Gel===
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* Errors:
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: Scun9-F-HEX: only had ~7 µL left
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{| {{table}} style="text-align: center"
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: Scun19-R-pig: only had ~46 µL left
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|-
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: added 50 + 30 = 80 µL H<sub>2</sub>O to make up for volume lost
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! Pour !! Load !! Run
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|-
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| 270 mL 1x TAE + 5.4 g agarose || 2 µL gel load dye + 4 µL PCR product || 160 V
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| 27 µL GelRed || 6 µL ladder || 45 minutes
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|}
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[[Image:20120110_Gela.png|800 px]]
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* mispriming everywhere - primer pair not usable for CNHY
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* lots of mispriming, but may be able to use conditions indicated by orange face ([[Image:orangefrownyface.png|20 px]]), if needed, under stringent conditions
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* favorable conditions indicated by [[Image:greenhappyface.png|25 px]]
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* [[Image:20121018_PCRGrid.png|800 px]]

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Contents


SCOC Batch02

  • Samples = USGS SYR & LOM + Clark HOL & RAJ


PCR

  • MP1-G


  • Errors:
Scun9-F-HEX: only had ~7 µL left
Scun19-R-pig: only had ~46 µL left
added 50 + 30 = 80 µL H2O to make up for volume lost




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