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| | ==SCOC Batch 2== | | ==SCOC Batch 2== |
| | + | * Samples = USGS SYR & LOM + Clark HOL & RAJ |
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| | ===Gel=== | | ===Gel=== |
| | + | * [[Image:20121025_Gel.png|800 px]] |
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| - | {| {{table}} style="text-align: center"
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| - | ! Pour !! Load !! Run
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| - | | 270 mL 1x TAE + 5.4 g agarose || 2 µL gel load dye + 4 µL PCR product || 160 V
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| - | | 27 µL GelRed || 6 µL ladder || 45 minutes
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| - | |}
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| - | [[Image:20120110_Gela.png|800 px]]
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| - | * mispriming everywhere - primer pair not usable for CNHY
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| - | * lots of mispriming, but may be able to use conditions indicated by orange face ([[Image:orangefrownyface.png|20 px]]), if needed, under stringent conditions
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| - | * favorable conditions indicated by [[Image:greenhappyface.png|25 px]]
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| | + | * At least one sample from each PCR showed bands; water samples showed no amplification. Send it. |
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Revision as of 19:00, 25 October 2012
 Tara's Lab Notebook
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SCOC Batch 2
- Samples = USGS SYR & LOM + Clark HOL & RAJ
Gel
- At least one sample from each PCR showed bands; water samples showed no amplification. Send it.
|
Tara's Lab Notebook
