User:Tara K. Luckau/Notebook/Team ConGen/2012/10/29

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(Autocreate 2012/10/29 Entry for User:Tara_K._Luckau/Notebook/Team_ConGen)
Current revision (19:06, 14 November 2012) (view source)
 
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==CNTI Acos5==
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==SCOC Batch02==
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===PCR===
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===Fragment Analysis Submission===
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* [[Image:20120110_PCRa.png|800 px]]
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===Gel===
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* 3µL MP1-B + 3µL MP1-G + 3µL MP1-Y
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{| {{table}} style="text-align: center"
 
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|-
 
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! Pour !! Load !! Run
 
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|-
 
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| 270 mL 1x TAE + 5.4 g agarose || 2 µL gel load dye + 4 µL PCR product || 160 V
 
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|-
 
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| 27 µL GelRed || 6 µL ladder || 45 minutes
 
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|}
 
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:: [[Image:20121029_Frag.png|900 px]]
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[[Image:20120110_Gela.png|800 px]]
 
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* mispriming everywhere - primer pair not usable for CNHY
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* FedEx Tracking# [http://www.fedex.com/Tracking?language=english&cntry_code=us&tracknumbers=801124184839 801124184839]
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* lots of mispriming, but may be able to use conditions indicated by orange face ([[Image:orangefrownyface.png|20 px]]), if needed, under stringent conditions
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:: [[Image:20121029_FedEx.png|700 px]]
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* favorable conditions indicated by [[Image:greenhappyface.png|25 px]]
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* UAGC Submission# QMCC & SYAZ
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* FedEx picked up 2:59pm, 29 Octover
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* FedEx delivered 9:14am, 30 October
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* UAGC received 3:26pm, 30 October
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* UAGC completed 10:10am, 31 October

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Contents


SCOC Batch02

Fragment Analysis Submission

  • 3µL MP1-B + 3µL MP1-G + 3µL MP1-Y




  • UAGC Submission# QMCC & SYAZ


  • FedEx picked up 2:59pm, 29 Octover
  • FedEx delivered 9:14am, 30 October
  • UAGC received 3:26pm, 30 October
  • UAGC completed 10:10am, 31 October



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