User:Tara K. Luckau/Notebook/Team ConGen/2012/11/08

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(Autocreate 2012/11/08 Entry for User:Tara_K._Luckau/Notebook/Team_ConGen)
Current revision (19:08, 14 November 2012) (view source)
 
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==CNTI Acos5==
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==SCOC Batch03==
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* Samples = USGS TP & CPN + Clark TP & SYR
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===PCR===
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===Gel===
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* [[Image:20120110_PCRa.png|800 px]]
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* [[Image:20121108_Gel.png|800 px]]
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===Gel===
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* At least one sample from each PCR showed bands; water samples showed no amplification. Send it.
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===Fragment Analysis Submission===
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* 3µL MP1-B + 3µL MP1-G + 3µL MP1-Y
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:: [[Image:20121108_Frag.png|900 px]]
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{| {{table}} style="text-align: center"
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* FedEx Tracking# [http://www.fedex.com/Tracking?language=english&cntry_code=us&tracknumbers=801124184828 801124184828]
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|-
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:: [[Image:20121108_FedEx.png|700 px]]
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! Pour !! Load !! Run
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|-
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| 270 mL 1x TAE + 5.4 g agarose || 2 µL gel load dye + 4 µL PCR product || 160 V
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| 27 µL GelRed || 6 µL ladder || 45 minutes
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|}
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[[Image:20120110_Gela.png|800 px]]
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* UAGC Submission# TZNF & WKAY
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* mispriming everywhere - primer pair not usable for CNHY
 
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* lots of mispriming, but may be able to use conditions indicated by orange face ([[Image:orangefrownyface.png|20 px]]), if needed, under stringent conditions
 
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* favorable conditions indicated by [[Image:greenhappyface.png|25 px]]
 
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* FedEx picked up 2:48pm, 8 November
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* FedEx delivered 9:16am, 9 November
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* UAGC received 10:55am, 9 November
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* UAGC completed 1:54pm, 9 November

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Contents


SCOC Batch03

  • Samples = USGS TP & CPN + Clark TP & SYR


Gel


  • At least one sample from each PCR showed bands; water samples showed no amplification. Send it.


Fragment Analysis Submission

  • 3µL MP1-B + 3µL MP1-G + 3µL MP1-Y




  • UAGC Submission# TZNF & WKAY


  • FedEx picked up 2:48pm, 8 November
  • FedEx delivered 9:16am, 9 November
  • UAGC received 10:55am, 9 November
  • UAGC completed 1:54pm, 9 November



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