User:Tara K. Luckau/Notebook/Team ConGen/2012/11/14: Difference between revisions

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==CNTI Acos5==
==CNHY MP1a/b==
* optimize for marker peak height - vary each primer
* CNHY MP1a: increase Ai5013, Ai5043, Ai5071
* CNHY MP1a: decrease Acos5
* CNHY MP1b: increase Ai5057, Ai5062




===PCR===
===PCR===
* [[Image:20120110_PCRa.png|800 px]]
* [[Image:20121114_PCRa.png|800 px]]
 
* [[Image:20121114_PCRb.png|800 px]]
 
* [[Image:20121114_PCRc.png|800 px]]
===Gel===
 
{| {{table}} style="text-align: center"
|-
! Pour !! Load !! Run
|-
| 270 mL 1x TAE + 5.4 g agarose || 2 µL gel load dye + 4 µL PCR product || 160 V
|-
| 27 µL GelRed || 6 µL ladder || 45 minutes
|}
 
 
[[Image:20120110_Gela.png|800 px]]
 
* mispriming everywhere - primer pair not usable for CNHY
* lots of mispriming, but may be able to use conditions indicated by orange face ([[Image:orangefrownyface.png|20 px]]), if needed, under stringent conditions
* favorable conditions indicated by [[Image:greenhappyface.png|25 px]]
 




Revision as of 16:52, 14 November 2012

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CNHY MP1a/b

  • optimize for marker peak height - vary each primer
  • CNHY MP1a: increase Ai5013, Ai5043, Ai5071
  • CNHY MP1a: decrease Acos5
  • CNHY MP1b: increase Ai5057, Ai5062


PCR



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