User:Tara K. Luckau/Notebook/Team ConGen/2012/11/14

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(Autocreate 2012/11/14 Entry for User:Tara_K._Luckau/Notebook/Team_ConGen)
Current revision (12:40, 20 February 2013) (view source)
 
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==CNTI Acos5==
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==ASHY MP1a/b Optimization==
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* optimize for marker peak height - vary each primer (based on results from 23 July 2012)
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* ASHY MP1a: increase Ai5013, Ai5043, Ai5071
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* ASHY MP1a: decrease Acos5
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* ASHY MP1b: increase Ai5057, Ai5062
===PCR===
===PCR===
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* [[Image:20120110_PCRa.png|800 px]]
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====ASHY MP1a: increase Ai5013, Ai5043, Ai5071====
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:: [[Image:20121114_PCRa.png|800 px]]
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===Gel===
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====ASHY MP1a: decrease Acos5====
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:: [[Image:20121114_PCRb.png|800 px]]
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{| {{table}} style="text-align: center"
 
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|-
 
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! Pour !! Load !! Run
 
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|-
 
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| 270 mL 1x TAE + 5.4 g agarose || 2 µL gel load dye + 4 µL PCR product || 160 V
 
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|-
 
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| 27 µL GelRed || 6 µL ladder || 45 minutes
 
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|}
 
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[[Image:20120110_Gela.png|800 px]]
 
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* mispriming everywhere - primer pair not usable for CNHY
 
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* lots of mispriming, but may be able to use conditions indicated by orange face ([[Image:orangefrownyface.png|20 px]]), if needed, under stringent conditions
 
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* favorable conditions indicated by [[Image:greenhappyface.png|25 px]]
 
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====ASHY MP1b: increase Ai5057, Ai5062====
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:: [[Image:20121114_PCRc.png|800 px]]

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Contents


ASHY MP1a/b Optimization

  • optimize for marker peak height - vary each primer (based on results from 23 July 2012)
  • ASHY MP1a: increase Ai5013, Ai5043, Ai5071
  • ASHY MP1a: decrease Acos5
  • ASHY MP1b: increase Ai5057, Ai5062


PCR

ASHY MP1a: increase Ai5013, Ai5043, Ai5071


ASHY MP1a: decrease Acos5


ASHY MP1b: increase Ai5057, Ai5062



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