User:Tara K. Luckau/Notebook/Team ConGen/2012/11/14: Difference between revisions

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|style="background-color: #BCED91"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Tara's Lab Notebook</span>
|style="background-color: #BCED91"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Tara's Lab Notebook</span>
|style="background-color: #9DB68C" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #9DB68C" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}}
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==CNTI Acos5==
==ASHY MP1a/b Optimization==
* optimize for marker peak height - vary each primer (based on results from 23 July 2012)
* ASHY MP1a: increase Ai5013, Ai5043, Ai5071
* ASHY MP1a: decrease Acos5
* ASHY MP1b: increase Ai5057, Ai5062




===PCR===
===PCR===
* [[Image:20120110_PCRa.png|800 px]]
====ASHY MP1a: increase Ai5013, Ai5043, Ai5071====
:: [[Image:20121114_PCRa.png|800 px]]




===Gel===
====ASHY MP1a: decrease Acos5====
:: [[Image:20121114_PCRb.png|800 px]]


{| {{table}} style="text-align: center"
|-
! Pour !! Load !! Run
|-
| 270 mL 1x TAE + 5.4 g agarose || 2 µL gel load dye + 4 µL PCR product || 160 V
|-
| 27 µL GelRed || 6 µL ladder || 45 minutes
|}
[[Image:20120110_Gela.png|800 px]]
* mispriming everywhere - primer pair not usable for CNHY
* lots of mispriming, but may be able to use conditions indicated by orange face ([[Image:orangefrownyface.png|20 px]]), if needed, under stringent conditions
* favorable conditions indicated by [[Image:greenhappyface.png|25 px]]


====ASHY MP1b: increase Ai5057, Ai5062====
:: [[Image:20121114_PCRc.png|800 px]]




Latest revision as of 22:15, 26 September 2017

Tara's Lab Notebook Main project page
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ASHY MP1a/b Optimization

  • optimize for marker peak height - vary each primer (based on results from 23 July 2012)
  • ASHY MP1a: increase Ai5013, Ai5043, Ai5071
  • ASHY MP1a: decrease Acos5
  • ASHY MP1b: increase Ai5057, Ai5062


PCR

ASHY MP1a: increase Ai5013, Ai5043, Ai5071


ASHY MP1a: decrease Acos5


ASHY MP1b: increase Ai5057, Ai5062



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