User:Tara K. Luckau/Notebook/Team ConGen/2012/11/14 - Revision history

Tara K. Luckau at 16:40, 20 February 2013

Tara K. Luckau — Wed, 20 Feb 2013 16:40:00 GMT

←Older revision		Revision as of 16:40, 20 February 2013
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-	==~~CNHY~~ MP1a/b Optimization==	+	==ASHY MP1a/b Optimization==
	* optimize for marker peak height - vary each primer (based on results from 23 July 2012)		* optimize for marker peak height - vary each primer (based on results from 23 July 2012)
-	* ~~CNHY~~ MP1a: increase Ai5013, Ai5043, Ai5071	+	* ASHY MP1a: increase Ai5013, Ai5043, Ai5071
-	* ~~CNHY~~ MP1a: decrease Acos5	+	* ASHY MP1a: decrease Acos5
-	* ~~CNHY~~ MP1b: increase Ai5057, Ai5062	+	* ASHY MP1b: increase Ai5057, Ai5062


	===PCR===		===PCR===
-	====~~CNHY~~ MP1a: increase Ai5013, Ai5043, Ai5071====	+	====ASHY MP1a: increase Ai5013, Ai5043, Ai5071====
	:: [[Image:20121114_PCRa.png\|800 px]]		:: [[Image:20121114_PCRa.png\|800 px]]


-	====~~CNHY~~ MP1a: decrease Acos5====	+	====ASHY MP1a: decrease Acos5====
	:: [[Image:20121114_PCRb.png\|800 px]]		:: [[Image:20121114_PCRb.png\|800 px]]


-	====~~CNHY~~ MP1b: increase Ai5057, Ai5062====	+	====ASHY MP1b: increase Ai5057, Ai5062====
	:: [[Image:20121114_PCRc.png\|800 px]]		:: [[Image:20121114_PCRc.png\|800 px]]

Tara K. Luckau at 22:16, 27 November 2012

Tara K. Luckau — Tue, 27 Nov 2012 22:16:28 GMT

←Older revision		Revision as of 22:16, 27 November 2012
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	===PCR===		===PCR===
-	* [[Image:20121114_PCRa.png\|800 px]]	+	====CNHY MP1a: increase Ai5013, Ai5043, Ai5071====
		+	:: [[Image:20121114_PCRa.png\|800 px]]


-	* [[Image:20121114_PCRb.png\|800 px]]	+	====CNHY MP1a: decrease Acos5====
		+	:: [[Image:20121114_PCRb.png\|800 px]]


-	* [[Image:20121114_PCRc.png\|800 px]]	+	====CNHY MP1b: increase Ai5057, Ai5062====
		+	:: [[Image:20121114_PCRc.png\|800 px]]

Tara K. Luckau at 22:14, 27 November 2012

Tara K. Luckau — Tue, 27 Nov 2012 22:14:06 GMT

←Older revision		Revision as of 22:14, 27 November 2012
Line 9:		Line 9:


-	==CNHY MP1a/b==	+	==CNHY MP1a/b Optimization==
-	* optimize for marker peak height - vary each primer	+	* optimize for marker peak height - vary each primer (based on results from 23 July 2012)
	* CNHY MP1a: increase Ai5013, Ai5043, Ai5071		* CNHY MP1a: increase Ai5013, Ai5043, Ai5071
	* CNHY MP1a: decrease Acos5		* CNHY MP1a: decrease Acos5
Line 18:		Line 18:
	===PCR===		===PCR===
	* [[Image:20121114_PCRa.png\|800 px]]		* [[Image:20121114_PCRa.png\|800 px]]
		+
		+
	* [[Image:20121114_PCRb.png\|800 px]]		* [[Image:20121114_PCRb.png\|800 px]]
		+
		+
	* [[Image:20121114_PCRc.png\|800 px]]		* [[Image:20121114_PCRc.png\|800 px]]

Tara K. Luckau at 23:52, 14 November 2012

Tara K. Luckau — Wed, 14 Nov 2012 23:52:07 GMT

←Older revision		Revision as of 23:52, 14 November 2012
Line 9:		Line 9:


-	==~~CNTI Acos5~~==	+	==CNHY MP1a/b==
		+	* optimize for marker peak height - vary each primer
		+	* CNHY MP1a: increase Ai5013, Ai5043, Ai5071
		+	* CNHY MP1a: decrease Acos5
		+	* CNHY MP1b: increase Ai5057, Ai5062


	===PCR===		===PCR===
-	* [[Image:~~20120110_PCRa~~.png\|800 px]]	+	* [[Image:20121114_PCRa.png\|800 px]]
-		+	* [[Image:20121114_PCRb.png\|800 px]]
-		+	* [[Image:20121114_PCRc.png\|800 px]]
-	~~===Gel===~~	+
-		+
-	~~{\| {{table}} style="text-align: center"~~	+
-	\|-	+
-	~~! Pour !! Load !! Run~~	+
-	\|-	+
-	~~\| 270 mL 1x TAE + 5.4 g agarose \|\| 2 µL gel load dye + 4 µL PCR product \|\| 160 V~~	+
-	\|-	+
-	~~\| 27 µL GelRed \|\| 6 µL ladder \|\| 45 minutes~~	+
-	\|}	+
-		+
-		+
-	[[Image:~~20120110_Gela~~.png\|800 px]]	+
-		+
-	* mispriming everywhere - primer pair not usable for CNHY	+
-	* ~~lots of mispriming, but may be able to use conditions indicated by orange face (~~[[Image:~~orangefrownyface~~.png\|20 px]]~~), if needed, under stringent conditions~~	+
-	* favorable conditions indicated by [[Image:greenhappyface.png\|25 px]]	+
-		+

Tara K. Luckau: Autocreate 2012/11/14 Entry for User:Tara_K._Luckau/Notebook/Team_ConGen

Tara K. Luckau — Wed, 14 Nov 2012 23:40:59 GMT

Autocreate 2012/11/14 Entry for User:Tara_K._Luckau/Notebook/Team_ConGen

New page

{|{{table}} width="800"
|-
|style="background-color: #BCED91"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Tara's Lab Notebook</span>
|style="background-color: #9DB68C" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>      </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|-
| colspan="2"|

__TOC__

==CNTI Acos5==

===PCR===
* [[Image:20120110_PCRa.png|800 px]]

===Gel===

{| {{table}} style="text-align: center"
|-
! Pour !! Load !! Run
|-
| 270 mL 1x TAE + 5.4 g agarose || 2 µL gel load dye + 4 µL PCR product || 160 V
|-
| 27 µL GelRed || 6 µL ladder || 45 minutes
|}

[[Image:20120110_Gela.png|800 px]]

* mispriming everywhere - primer pair not usable for CNHY
* lots of mispriming, but may be able to use conditions indicated by orange face ([[Image:orangefrownyface.png|20 px]]), if needed, under stringent conditions
* favorable conditions indicated by [[Image:greenhappyface.png|25 px]]


|}

__NOTOC__