User:Tara K. Luckau/Notebook/Team ConGen/2012/11/15

(Difference between revisions)

Revision as of 19:21, 15 November 2012

Tara's Lab Notebook

At least one sample from each PCR showed bands; water samples showed no amplification. Send it.

@@ Line 9: / Line 9: @@
-==CNTI Acos5==
+==SCOC Batch03==
+* Samples = USGS TP & CPN + Clark TP & SYR
-===PCR===
+===Gel===
-* [[Image:20120110_PCRa.png|800 px]]
+* [[Image:20121108_Gel.png|800 px]]
-===Gel===
+* At least one sample from each PCR showed bands; water samples showed no amplification. Send it.
+===Fragment Analysis Submission===
+* 3µL MP1-B + 3µL MP1-G + 3µL MP1-Y
+:: [[Image:20121108_Frag.png|900 px]]
+* FedEx Tracking# [http://www.fedex.com/Tracking?language=english&cntry_code=us&tracknumbers=801124184828 801124184828]
+:: [[Image:20121108_FedEx.png|700 px]]
-{| {{table}} style="text-align: center"
-|-
-! Pour !! Load !! Run
-|-
-| 270 mL 1x TAE + 5.4 g agarose || 2 µL gel load dye + 4 µL PCR product || 160 V
-|-
-| 27 µL GelRed || 6 µL ladder || 45 minutes
-|}
+* UAGC Submission# TZNF & WKAY
-[[Image:20120110_Gela.png|800 px]]
-* mispriming everywhere - primer pair not usable for CNHY
+* FedEx picked up 2:48pm, 8 November
-* lots of mispriming, but may be able to use conditions indicated by orange face ([[Image:orangefrownyface.png|20 px]]), if needed, under stringent conditions
+* FedEx delivered 9:16am, 9 November
-* favorable conditions indicated by [[Image:greenhappyface.png|25 px]]
+* UAGC received 10:55am, 9 November
+* UAGC completed 1:54pm, 9 November