User:Tara K. Luckau/Notebook/Team ConGen/2012/11/15

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==SCOC Batch03==
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==CNHY MP1a/b Optimization==
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* Samples = USGS TP & CPN + Clark TP & SYR
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* optimize for marker peak height - vary each primer (based on results from 23 July 2012)
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* CNHY MP1a: increase Ai5013, Ai5043, Ai5071
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* CNHY MP1a: decrease Acos5
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* CNHY MP1b: increase Ai5057, Ai5062
===Gel===
===Gel===
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* [[Image:20121108_Gel.png|800 px]]
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* too lazy to do confirmation gel
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* At least one sample from each PCR showed bands; water samples showed no amplification. Send it.
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* 3µL MP1-B + 3µL MP1-G + 3µL MP1-Y
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* UAGC Submission# YFPQ
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:: [[Image:20121115_Frag.png|900 px]]
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:: [[Image:20121108_Frag.png|900 px]]
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* FedEx Tracking# [http://www.fedex.com/Tracking?language=english&cntry_code=us&tracknumbers=801124184828 801124184828]
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:: [[Image:20121108_FedEx.png|700 px]]
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* UAGC Submission# TZNF & WKAY
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* FedEx Tracking# [http://www.fedex.com/Tracking?language=english&cntry_code=us&tracknumbers=801124184817 801124184817]
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:: [[Image:20121115_FedEx.png|700 px]]
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* FedEx picked up 2:48pm, 8 November
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* FedEx picked up 3:01pm, 15 November
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* FedEx delivered 9:16am, 9 November
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* FedEx delivered 9:23am, 16 November
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* UAGC received 10:55am, 9 November
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* UAGC received 12:08pm, 16 November
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* UAGC completed 1:54pm, 9 November
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* UAGC completed 11:31am, 19 November

Current revision

Tara's Lab Notebook Main project page
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Contents


CNHY MP1a/b Optimization

  • optimize for marker peak height - vary each primer (based on results from 23 July 2012)
  • CNHY MP1a: increase Ai5013, Ai5043, Ai5071
  • CNHY MP1a: decrease Acos5
  • CNHY MP1b: increase Ai5057, Ai5062


Gel

  • too lazy to do confirmation gel


Fragment Analysis Submission

  • UAGC Submission# YFPQ



  • FedEx picked up 3:01pm, 15 November
  • FedEx delivered 9:23am, 16 November
  • UAGC received 12:08pm, 16 November
  • UAGC completed 11:31am, 19 November




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