User:Tara K. Luckau/Notebook/Team ConGen/2012/11/15

Current revision

Tara's Lab Notebook

optimize for marker peak height - vary each primer (based on results from 23 July 2012)
CNHY MP1a: increase Ai5013, Ai5043, Ai5071
CNHY MP1a: decrease Acos5
CNHY MP1b: increase Ai5057, Ai5062

@@ Line 9: / Line 9: @@
-==CNTI Acos5==
+==CNHY MP1a/b Optimization==
+* optimize for marker peak height - vary each primer (based on results from 23 July 2012)
+* CNHY MP1a: increase Ai5013, Ai5043, Ai5071
+* CNHY MP1a: decrease Acos5
+* CNHY MP1b: increase Ai5057, Ai5062
-===PCR===
+===Gel===
-* [[Image:20120110_PCRa.png|800 px]]
+* too lazy to do confirmation gel
-===Gel===
+===Fragment Analysis Submission===
+* UAGC Submission# YFPQ
+:: [[Image:20121115_Frag.png|900 px]]
-{| {{table}} style="text-align: center"
-|-
-! Pour !! Load !! Run
-|-
-| 270 mL 1x TAE + 5.4 g agarose || 2 µL gel load dye + 4 µL PCR product || 160 V
-|-
-| 27 µL GelRed || 6 µL ladder || 45 minutes
-|}
+* FedEx Tracking# [http://www.fedex.com/Tracking?language=english&cntry_code=us&tracknumbers=801124184817 801124184817]
+:: [[Image:20121115_FedEx.png|700 px]]
-[[Image:20120110_Gela.png|800 px]]
-* mispriming everywhere - primer pair not usable for CNHY
+* FedEx picked up 3:01pm, 15 November
-* lots of mispriming, but may be able to use conditions indicated by orange face ([[Image:orangefrownyface.png|20 px]]), if needed, under stringent conditions
+* FedEx delivered 9:23am, 16 November
-* favorable conditions indicated by [[Image:greenhappyface.png|25 px]]
+* UAGC received 12:08pm, 16 November
+* UAGC completed 11:31am, 19 November