User:Tara K. Luckau/Notebook/Team ConGen/2012/11/15 - Revision history

Tara K. Luckau at 22:18, 27 November 2012

Tara K. Luckau — Tue, 27 Nov 2012 22:18:12 GMT

←Older revision		Revision as of 22:18, 27 November 2012
Line 10:		Line 10:

	==CNHY MP1a/b Optimization==		==CNHY MP1a/b Optimization==
-	* optimize for marker peak height - vary each primer	+	* optimize for marker peak height - vary each primer (based on results from 23 July 2012)
	* CNHY MP1a: increase Ai5013, Ai5043, Ai5071		* CNHY MP1a: increase Ai5013, Ai5043, Ai5071
	* CNHY MP1a: decrease Acos5		* CNHY MP1a: decrease Acos5

Tara K. Luckau at 22:17, 27 November 2012

Tara K. Luckau — Tue, 27 Nov 2012 22:17:15 GMT

←Older revision		Revision as of 22:17, 27 November 2012
Line 23:		Line 23:


		+	* UAGC Submission# YFPQ
	:: [[Image:20121115_Frag.png\|900 px]]		:: [[Image:20121115_Frag.png\|900 px]]

Line 28:		Line 29:
	* FedEx Tracking# [http://www.fedex.com/Tracking?language=english&cntry_code=us&tracknumbers=801124184817 801124184817]		* FedEx Tracking# [http://www.fedex.com/Tracking?language=english&cntry_code=us&tracknumbers=801124184817 801124184817]
	:: [[Image:20121115_FedEx.png\|700 px]]		:: [[Image:20121115_FedEx.png\|700 px]]
-
-
-	* UAGC Submission# YFPQ

Tara K. Luckau at 22:13, 27 November 2012

Tara K. Luckau — Tue, 27 Nov 2012 22:13:01 GMT

←Older revision		Revision as of 22:13, 27 November 2012
Line 36:		Line 36:
	* FedEx delivered 9:23am, 16 November		* FedEx delivered 9:23am, 16 November
	* UAGC received 12:08pm, 16 November		* UAGC received 12:08pm, 16 November
-	* UAGC completed pm, 16 November	+	* UAGC completed 11:31am, 19 November

Tara K. Luckau at 20:12, 16 November 2012

Tara K. Luckau — Fri, 16 Nov 2012 20:12:48 GMT

←Older revision		Revision as of 20:12, 16 November 2012
Line 35:		Line 35:
	* FedEx picked up 3:01pm, 15 November		* FedEx picked up 3:01pm, 15 November
	* FedEx delivered 9:23am, 16 November		* FedEx delivered 9:23am, 16 November
-	* UAGC received am, 16 November	+	* UAGC received 12:08pm, 16 November
	* UAGC completed pm, 16 November		* UAGC completed pm, 16 November

Tara K. Luckau at 18:38, 16 November 2012

Tara K. Luckau — Fri, 16 Nov 2012 18:38:36 GMT

←Older revision		Revision as of 18:38, 16 November 2012
Line 9:		Line 9:


-	==CNHY MP1a/b==	+	==CNHY MP1a/b Optimization==
	* optimize for marker peak height - vary each primer		* optimize for marker peak height - vary each primer
	* CNHY MP1a: increase Ai5013, Ai5043, Ai5071		* CNHY MP1a: increase Ai5013, Ai5043, Ai5071
Line 33:		Line 33:


-	* FedEx picked up pm, 15 November	+	* FedEx picked up 3:01pm, 15 November
-	* FedEx delivered am, 16 November	+	* FedEx delivered 9:23am, 16 November
	* UAGC received am, 16 November		* UAGC received am, 16 November
	* UAGC completed pm, 16 November		* UAGC completed pm, 16 November

Tara K. Luckau at 23:30, 15 November 2012

Tara K. Luckau — Thu, 15 Nov 2012 23:30:31 GMT

←Older revision		Revision as of 23:30, 15 November 2012
Line 9:		Line 9:


-	==~~SCOC Batch03~~==	+	==CNHY MP1a/b==
-	* ~~Samples = USGS TP & CPN + Clark TP & SYR~~	+	* optimize for marker peak height - vary each primer
		+	* CNHY MP1a: increase Ai5013, Ai5043, Ai5071
		+	* CNHY MP1a: decrease Acos5
		+	* CNHY MP1b: increase Ai5057, Ai5062


	===Gel===		===Gel===
-	* ~~[[Image:20121108_Gel.png\|800 px]]~~	+	* too lazy to do confirmation gel
-		+
-		+
-	* At least one sample from each PCR showed bands; water samples showed no amplification. Send it.	+


Line 23:		Line 23:


-	* 3µL MP1-B + 3µL MP1-G + 3µL MP1-Y	+	:: [[Image:20121115_Frag.png\|900 px]]
-		+
-		+
-	:: [[Image:~~20121108_Frag~~.png\|900 px]]	+


-	* FedEx Tracking# [http://www.fedex.com/Tracking?language=english&cntry_code=us&tracknumbers=~~801124184828 801124184828~~]	+	* FedEx Tracking# [http://www.fedex.com/Tracking?language=english&cntry_code=us&tracknumbers=801124184817 801124184817]
-	:: [[Image:~~20121108_FedEx~~.png\|700 px]]	+	:: [[Image:20121115_FedEx.png\|700 px]]


-	* UAGC Submission# ~~TZNF & WKAY~~	+	* UAGC Submission# YFPQ


-	* FedEx picked up ~~2:48pm~~, 8 November	+	* FedEx picked up pm, 15 November
-	* FedEx delivered ~~9:16am~~, 9 November	+	* FedEx delivered am, 16 November
-	* UAGC received ~~10:55am~~, 9 November	+	* UAGC received am, 16 November
-	* UAGC completed ~~1:54pm~~, 9 November	+	* UAGC completed pm, 16 November

Tara K. Luckau at 23:21, 15 November 2012

Tara K. Luckau — Thu, 15 Nov 2012 23:21:38 GMT

←Older revision		Revision as of 23:21, 15 November 2012
Line 9:		Line 9:


-	==~~CNTI Acos5~~==	+	==SCOC Batch03==
		+	* Samples = USGS TP & CPN + Clark TP & SYR


-	===~~PCR~~===	+	===Gel===
-	* [[Image:~~20120110_PCRa~~.png\|800 px]]	+	* [[Image:20121108_Gel.png\|800 px]]


-	===~~Gel~~===	+	* At least one sample from each PCR showed bands; water samples showed no amplification. Send it.
		+
		+
		+	===Fragment Analysis Submission===
		+
		+
		+	* 3µL MP1-B + 3µL MP1-G + 3µL MP1-Y
		+
		+
		+	:: [[Image:20121108_Frag.png\|900 px]]
		+
		+
		+	* FedEx Tracking# [http://www.fedex.com/Tracking?language=english&cntry_code=us&tracknumbers=801124184828 801124184828]
		+	:: [[Image:20121108_FedEx.png\|700 px]]

-	~~{\| {{table}} style="text-align: center"~~
-	\|-
-	~~! Pour !! Load !! Run~~
-	\|-
-	~~\| 270 mL 1x TAE + 5.4 g agarose \|\| 2 µL gel load dye + 4 µL PCR product \|\| 160 V~~
-	\|-
-	~~\| 27 µL GelRed \|\| 6 µL ladder \|\| 45 minutes~~
-	\|}

		+	* UAGC Submission# TZNF & WKAY

-	~~[[Image:20120110_Gela.png\|800 px]]~~

-	* ~~mispriming everywhere - primer pair not usable for CNHY~~	+	* FedEx picked up 2:48pm, 8 November
-	* ~~lots of mispriming~~, ~~but may be able to use conditions indicated by orange face ([[Image~~:~~orangefrownyface.png\|20 px]])~~, ~~if needed, under stringent conditions~~	+	* FedEx delivered 9:16am, 9 November
-	* ~~favorable conditions indicated by [[Image~~:~~greenhappyface.png\|25 px]]~~	+	* UAGC received 10:55am, 9 November
		+	* UAGC completed 1:54pm, 9 November

Tara K. Luckau: Autocreate 2012/11/15 Entry for User:Tara_K._Luckau/Notebook/Team_ConGen

Tara K. Luckau — Thu, 15 Nov 2012 23:20:52 GMT

Autocreate 2012/11/15 Entry for User:Tara_K._Luckau/Notebook/Team_ConGen

New page

{|{{table}} width="800"
|-
|style="background-color: #BCED91"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Tara's Lab Notebook</span>
|style="background-color: #9DB68C" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>      </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|-
| colspan="2"|

__TOC__

==CNTI Acos5==

===PCR===
* [[Image:20120110_PCRa.png|800 px]]

===Gel===

{| {{table}} style="text-align: center"
|-
! Pour !! Load !! Run
|-
| 270 mL 1x TAE + 5.4 g agarose || 2 µL gel load dye + 4 µL PCR product || 160 V
|-
| 27 µL GelRed || 6 µL ladder || 45 minutes
|}

[[Image:20120110_Gela.png|800 px]]

* mispriming everywhere - primer pair not usable for CNHY
* lots of mispriming, but may be able to use conditions indicated by orange face ([[Image:orangefrownyface.png|20 px]]), if needed, under stringent conditions
* favorable conditions indicated by [[Image:greenhappyface.png|25 px]]


|}

__NOTOC__