User:Tara K. Luckau/Notebook/Team ConGen/2012/11/27: Difference between revisions

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* CNHY MP1a: decrease Acos5
* CNHY MP1a: decrease Acos5
* CNHY MP1b: increase Ai5057, Ai5062
* CNHY MP1b: increase Ai5057, Ai5062


===Frag Results===
===Frag Results===

Revision as of 15:52, 27 November 2012

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CNHY MP1a/b Optimization

  • optimize for marker peak height - vary each primer (based on results from 23 July 2012)
  • CNHY MP1a: increase Ai5013, Ai5043, Ai5071
  • CNHY MP1a: decrease Acos5
  • CNHY MP1b: increase Ai5057, Ai5062


Frag Results

  • UAGC submission# YFPQ


MP1a

Acos3
  • 23 July 2012: no changes needed
  • this time:
still fine, though seems to decrease with increasing Acos5 as well as Ai5071
Acos5
  • 23 July 2012: too high
  • this time:
0.04 = too low
0.06 = inconclusive (failures)
0.08 = too high
Ai5013
  • 23 July 2012: too low
  • this time:
second peak never got above 250, regardless of concentration
however, in Acos5 assay, noticed second peak reached 400 intensity as Acos5 decreased
Ai5043
  • 23 July 2012: did not amplify
  • this time: did not amplify
remove from multiplex
Ai5071
  • 23 July 2012: too low
  • this time:
best at 0.24, 0.28, 0.32 (200-300 intensity only)


MP1b

Ai5033
  • 23 July 2012: no changes needed
  • this time:
still fine (no amp for any markers in Ai5057 assay)
Ai5035
  • 23 July 2012: no changes needed
  • this time:
a bit low in Ai5062 assay (no amp for any markers in Ai5057 assay)
Ai5037
  • 23 July 2012: no changes needed
  • this time:
still fine (no amp for any markers in Ai5057 assay)
Ai5057
  • 23 July 2012: did not amplify
  • this time: did not amplify
remove from multiplex
Ai5062
  • 23 July 2012: too low
  • this time:
best at 0.48 (400 & 6000 intensity)




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