User:Tara K. Luckau/Notebook/Team ConGen/2012/11/27

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
Line 18: Line 18:
* UAGC submission# YFPQ
* UAGC submission# YFPQ
 +
 +
====MP1a====
 +
=====Acos3=====
 +
* 23 July 2012: no changes needed
 +
* this time:
 +
: still fine, though seems to decrease with increasing Acos5 as well as Ai5071
 +
=====Acos5=====
 +
* 23 July 2012: too high
 +
* this time:
 +
: 0.04 = too low
 +
: 0.06 = inconclusive (failures)
 +
: 0.08 = too high
 +
=====Ai5013=====
 +
* 23 July 2012: too low
 +
* this time:
 +
: second peak never got above 250, regardless of concentration
 +
: however, in Acos5 assay, noticed second peak reached 400 intensity as Acos5 decreased
 +
=====Ai5043=====
 +
* 23 July 2012: did not amplify
 +
* this time: did not amplify
 +
: remove from multiplex
 +
=====Ai5071=====
 +
* 23 July 2012: too low
 +
* this time:
 +
: best at 0.24, 0.28, 0.32 (200-300 intensity only)
 +
 +
 +
====MP1b====
 +
=====Ai5033=====
 +
* 23 July 2012: no changes needed
 +
* this time:
 +
: still fine (no amp for any markers in Ai5057 assay)
 +
=====Ai5035=====
 +
* 23 July 2012: no changes needed
 +
* this time:
 +
: a bit low in Ai5062 assay (no amp for any markers in Ai5057 assay)
 +
=====Ai5037=====
 +
* 23 July 2012: no changes needed
 +
* this time:
 +
: still fine (no amp for any markers in Ai5057 assay)
 +
=====Ai5057=====
 +
* 23 July 2012: did not amplify
 +
* this time: did not amplify
 +
: remove from multiplex
 +
=====Ai5062=====
 +
* 23 July 2012: too low
 +
* this time:
 +
: best at 0.48 (400 & 6000 intensity)

Revision as of 18:49, 27 November 2012

Tara's Lab Notebook Main project page
Previous entry      Next entry

Contents


CNHY MP1a/b Optimization

  • optimize for marker peak height - vary each primer (based on results from 23 July 2012)
  • CNHY MP1a: increase Ai5013, Ai5043, Ai5071
  • CNHY MP1a: decrease Acos5
  • CNHY MP1b: increase Ai5057, Ai5062

Frag Results

  • UAGC submission# YFPQ


MP1a

Acos3
  • 23 July 2012: no changes needed
  • this time:
still fine, though seems to decrease with increasing Acos5 as well as Ai5071
Acos5
  • 23 July 2012: too high
  • this time:
0.04 = too low
0.06 = inconclusive (failures)
0.08 = too high
Ai5013
  • 23 July 2012: too low
  • this time:
second peak never got above 250, regardless of concentration
however, in Acos5 assay, noticed second peak reached 400 intensity as Acos5 decreased
Ai5043
  • 23 July 2012: did not amplify
  • this time: did not amplify
remove from multiplex
Ai5071
  • 23 July 2012: too low
  • this time:
best at 0.24, 0.28, 0.32 (200-300 intensity only)


MP1b

Ai5033
  • 23 July 2012: no changes needed
  • this time:
still fine (no amp for any markers in Ai5057 assay)
Ai5035
  • 23 July 2012: no changes needed
  • this time:
a bit low in Ai5062 assay (no amp for any markers in Ai5057 assay)
Ai5037
  • 23 July 2012: no changes needed
  • this time:
still fine (no amp for any markers in Ai5057 assay)
Ai5057
  • 23 July 2012: did not amplify
  • this time: did not amplify
remove from multiplex
Ai5062
  • 23 July 2012: too low
  • this time:
best at 0.48 (400 & 6000 intensity)




Personal tools