User:Tara K. Luckau/Notebook/Team ConGen/2013/02/08: Difference between revisions
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==CNTI | ==CNTI Cvanµ18== | ||
===Gel=== | |||
{| {{table}} style="text-align: center" | |||
|- | |||
! Pour !! Load !! Run | |||
|- | |||
| 270 mL 1x TAE + 5.4 g agarose || 2 µL gel load dye + 4 µL PCR product || 160 V | |||
|- | |||
| 27 µL GelRed || 6 µL ladder || 45 minutes | |||
|} | |||
[[Image:20130208_Gela.png|800 px]] | |||
* mispriming everywhere - primer pair not usable for CNHY | |||
* lots of mispriming, but may be able to use conditions indicated by orange face ([[Image:orangefrownyface.png|20 px]]), if needed, under stringent conditions | |||
* favorable conditions indicated by [[Image:greenhappyface.png|25 px]] | |||
==CNTI Cvanµ19== | |||
===PCR=== | ===PCR=== | ||
* [[Image: | * [[Image:20130208_PCRa.png|800 px]] | ||
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[[Image: | [[Image:20130208_Gelb.png|800 px]] | ||
* mispriming everywhere - primer pair not usable for CNHY | * mispriming everywhere - primer pair not usable for CNHY | ||
* lots of mispriming, but may be able to use conditions indicated by orange face ([[Image:orangefrownyface.png|20 px]]), if needed, under stringent conditions | * lots of mispriming, but may be able to use conditions indicated by orange face ([[Image:orangefrownyface.png|20 px]]), if needed, under stringent conditions | ||
* favorable conditions indicated by [[Image:greenhappyface.png|25 px]] | * favorable conditions indicated by [[Image:greenhappyface.png|25 px]] | ||
==CNTI Cvanµ23== | |||
===PCR=== | |||
* [[Image:20130208_PCRb.png|800 px]] | |||
Revision as of 14:12, 12 February 2013
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CNTI Cvanµ18Gel
CNTI Cvanµ19PCR
Gel
CNTI Cvanµ23PCR
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