User:Tara K. Luckau/Notebook/Team ConGen/2013/02/21

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(Autocreate 2013/02/21 Entry for User:Tara_K._Luckau/Notebook/Team_ConGen)
Line 9: Line 9:
-
==CNTI Acos5==
+
==re-do SCOC MP1 Batch 2==
===PCR===
===PCR===
-
* [[Image:20120110_PCRa.png|800 px]]
+
* MP1-G
 +
* [[Image:20130220_PCRg.png|800 px]]
-
===Gel===
+
* MP1-Y
-
 
+
* [[Image:20130220_PCRy.png|800 px]]
-
{| {{table}} style="text-align: center"
+
-
|-
+
-
! Pour !! Load !! Run
+
-
|-
+
-
| 270 mL 1x TAE + 5.4 g agarose || 2 µL gel load dye + 4 µL PCR product || 160 V
+
-
|-
+
-
| 27 µL GelRed || 6 µL ladder || 45 minutes
+
-
|}
+
-
 
+
-
 
+
-
[[Image:20120110_Gela.png|800 px]]
+
-
 
+
-
* mispriming everywhere - primer pair not usable for CNHY
+
-
* lots of mispriming, but may be able to use conditions indicated by orange face ([[Image:orangefrownyface.png|20 px]]), if needed, under stringent conditions
+
-
* favorable conditions indicated by [[Image:greenhappyface.png|25 px]]
+
 +
* [[Image:20130220_PCRPlate.png|800 px]]

Revision as of 17:48, 25 February 2013

Tara's Lab Notebook Main project page
Previous entry      Next entry

Contents


re-do SCOC MP1 Batch 2

PCR





Personal tools