User:Tara K. Luckau/Notebook/Team ConGen/2013/02/24: Difference between revisions

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==CNTI Acos5==
==SCOC MP1 Batch 2 Plate 1&2 (re-do)==
 
 
===PCR===
* [[Image:20120110_PCRa.png|800 px]]




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[[Image:20120110_Gela.png|800 px]]
[[Image:20130224_Gel.png|800 px]]


* mispriming everywhere - primer pair not usable for CNHY
* bands indicate successful PCR
* lots of mispriming, but may be able to use conditions indicated by orange face ([[Image:orangefrownyface.png|20 px]]), if needed, under stringent conditions
* no contamination in water
* favorable conditions indicated by [[Image:greenhappyface.png|25 px]]
* send to frag!




Revision as of 17:50, 24 February 2013

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SCOC MP1 Batch 2 Plate 1&2 (re-do)

Gel

Pour Load Run
270 mL 1x TAE + 5.4 g agarose 2 µL gel load dye + 4 µL PCR product 160 V
27 µL GelRed 6 µL ladder 45 minutes


  • bands indicate successful PCR
  • no contamination in water
  • send to frag!




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