User:Tara K. Luckau/Notebook/Team ConGen/2013/02/24

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(Autocreate 2013/02/24 Entry for User:Tara_K._Luckau/Notebook/Team_ConGen)
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==CNTI Acos5==
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==SCOC MP1 Batch 2 Plate 1&2==
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===PCR===
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* [[Image:20120110_PCRa.png|800 px]]
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[[Image:20120110_Gela.png|800 px]]
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[[Image:20130224_Gel.png|800 px]]
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* mispriming everywhere - primer pair not usable for CNHY
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* bands indicate successful PCR
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* lots of mispriming, but may be able to use conditions indicated by orange face ([[Image:orangefrownyface.png|20 px]]), if needed, under stringent conditions
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* no contamination in water
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* favorable conditions indicated by [[Image:greenhappyface.png|25 px]]
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* send to frag!

Revision as of 20:49, 24 February 2013

Tara's Lab Notebook Main project page
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Contents


SCOC MP1 Batch 2 Plate 1&2

Gel

Pour Load Run
270 mL 1x TAE + 5.4 g agarose 2 µL gel load dye + 4 µL PCR product 160 V
27 µL GelRed 6 µL ladder 45 minutes


  • bands indicate successful PCR
  • no contamination in water
  • send to frag!




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