User:Tara K. Luckau/Notebook/Team ConGen/2013/02/26: Difference between revisions

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==CNTI Acos5==
==SCOC MP2 Optimization==




===PCR===
* uniplex SW markers to see quality of peaks and range of alleles
* [[Image:20120110_PCRa.png|800 px]]
* multiplex SW markers as first glance of how multiplex behaves




===Gel===
===Gel===


{| {{table}} style="text-align: center"
|-
! Pour !! Load !! Run
|-
| 270 mL 1x TAE + 5.4 g agarose || 2 µL gel load dye + 4 µL PCR product || 160 V
|-
| 27 µL GelRed || 6 µL ladder || 45 minutes
|}


[[Image:20130226_Gel.png|800 px]]


[[Image:20120110_Gela.png|800 px]]
* stuff
 
* stuff
* mispriming everywhere - primer pair not usable for CNHY
* lots of mispriming, but may be able to use conditions indicated by orange face ([[Image:orangefrownyface.png|20 px]]), if needed, under stringent conditions
* favorable conditions indicated by [[Image:greenhappyface.png|25 px]]




Revision as of 13:29, 26 February 2013

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SCOC MP2 Optimization

  • uniplex SW markers to see quality of peaks and range of alleles
  • multiplex SW markers as first glance of how multiplex behaves


Gel

  • stuff
  • stuff




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