User:Tara K. Luckau/Notebook/Team ConGen/2013/02/26

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(Autocreate 2013/02/26 Entry for User:Tara_K._Luckau/Notebook/Team_ConGen)
Current revision (15:06, 1 March 2013) (view source)
 
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==CNTI Acos5==
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==SCOC MP2 Optimization==
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===PCR===
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* uniplex SW markers to see quality of peaks and range of alleles
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* [[Image:20120110_PCRa.png|800 px]]
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* multiplex SW markers as first glance of how multiplex behaves
===Gel===
===Gel===
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{| {{table}} style="text-align: center"
 
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|-
 
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! Pour !! Load !! Run
 
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|-
 
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| 270 mL 1x TAE + 5.4 g agarose || 2 µL gel load dye + 4 µL PCR product || 160 V
 
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|-
 
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| 27 µL GelRed || 6 µL ladder || 45 minutes
 
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|}
 
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[[Image:20130226_Gel.png|800 px]]
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[[Image:20120110_Gela.png|800 px]]
 
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* mispriming everywhere - primer pair not usable for CNHY
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* gel floated sideways, so it looks funny, but whatevs
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* lots of mispriming, but may be able to use conditions indicated by orange face ([[Image:orangefrownyface.png|20 px]]), if needed, under stringent conditions
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* clean bands in uniplexes
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* favorable conditions indicated by [[Image:greenhappyface.png|25 px]]
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* multiplex has amplification
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* send to frag!
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===Fragment Analysis Submission===
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:: [[Image:20130226_Frag.png|900 px]]
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* FedEx Tracking# [http://www.fedex.com/Tracking?language=english&cntry_code=us&tracknumbers=801124151778 801124151778]
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:: [[Image:20130226_FedEx.png|700 px]]
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* UAGC Submission# PPTX
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* FedEx picked up 2:44pm, 26 February
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* FedEx delivered 9:29am, 27 February
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* UAGC received 10:58pm, 27 February
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* UAGC completed 10:41am, 28 February

Current revision

Tara's Lab Notebook Main project page
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Contents


SCOC MP2 Optimization

  • uniplex SW markers to see quality of peaks and range of alleles
  • multiplex SW markers as first glance of how multiplex behaves


Gel


  • gel floated sideways, so it looks funny, but whatevs
  • clean bands in uniplexes
  • multiplex has amplification
  • send to frag!


Fragment Analysis Submission



  • UAGC Submission# PPTX


  • FedEx picked up 2:44pm, 26 February
  • FedEx delivered 9:29am, 27 February
  • UAGC received 10:58pm, 27 February
  • UAGC completed 10:41am, 28 February




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