User:Tara K. Luckau/Notebook/Team ConGen/2013/02/26

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Revision as of 16:28, 26 February 2013

Tara's Lab Notebook Main project page
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Contents


CNTI Acos5

PCR


Gel

Pour Load Run
270 mL 1x TAE + 5.4 g agarose 2 µL gel load dye + 4 µL PCR product 160 V
27 µL GelRed 6 µL ladder 45 minutes


  • mispriming everywhere - primer pair not usable for CNHY
  • lots of mispriming, but may be able to use conditions indicated by orange face (), if needed, under stringent conditions
  • favorable conditions indicated by




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