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 Tara's Lab Notebook
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SCOC MP2 Optimization
- uniplex SW markers to see quality of peaks and range of alleles
- multiplex SW markers as first glance of how multiplex behaves
Results
- Frag data: PPTX
- also using frag data from FHVZ (late November 2012)
- (apparently, I already did this PCR of uniplexing each SW marker, then throwing it all in together ... oops)
| Marker |
SYR Genotype |
TP3 Genotype |
Uniplex comments |
Multiplex comments
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| SW614-A1 (NED) |
410 415 |
[poop] |
tall @ 8000, very clean |
short @ 300, clean
|
| SW614-A4 (FAM) |
395 396 |
396 398 |
tall @ 15000 |
short @ 300
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| SW614-A6 (PET) |
[poop] |
255 275 |
tall @ 8000 |
good @ 1500
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| SW614-A7 (HEX) |
252 252 |
248 248 |
tall @ 14000 |
no amplification
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| SW614-B1 (HEX) |
316 316 |
299 316 |
tall @ 16000, one replicate of SYR poop |
good @ 1200
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| SW614-B6 (FAM) |
308 310 |
308 310 |
tall @ 11000 |
no amplification
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| SW614-B10 (FAM) |
188 194 |
186 198 |
tall @ 12000, one replicate of SYR violates slope rule |
no amplification
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| SW614-B12 (NED) |
285 285 |
255 274 |
tall @ 14000 |
good @ 2000
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Tara's Lab Notebook
