User:Tara K. Luckau/Notebook/Team ConGen/2013/02/27

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Contents


SCOC MP2 Optimization

  • uniplex SW markers to see quality of peaks and range of alleles
  • multiplex SW markers as first glance of how multiplex behaves


Results

  • Frag data: PPTX
  • also using frag data from FHVZ (late November 2012)
    • (apparently, I already did this PCR of uniplexing each SW marker, then throwing it all in together ... oops)


Marker SYR Genotype TP3 Genotype Uniplex comments Multiplex comments
SW614-A1 (NED) 410 415 [poop] tall @ 8000, very clean short @ 300, clean
SW614-A4 (FAM) 395 396 396 398 tall @ 15000 short @ 300
SW614-A6 (PET) [poop] 255 275 tall @ 8000 good @ 1500
SW614-A7 (HEX) 252 252 248 248 tall @ 14000 no amplification
SW614-B1 (HEX) 316 316 299 316 tall @ 16000, one replicate of SYR poop good @ 1200
SW614-B6 (FAM) 308 310 308 310 tall @ 11000 no amplification
SW614-B10 (FAM) 188 194 186 198 tall @ 12000, one replicate of SYR violates slope rule no amplification
SW614-B12 (NED) 285 285 255 274 tall @ 14000 good @ 2000


  • move forward with SW614-A4, SW614-B1 and SW614-B12 as SCOC MP2 (still have to consider sar80?)



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