User:Tara K. Luckau/Notebook/Team ConGen/2013/03/07: Difference between revisions

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==CNTI Acos5==
==SCOC MP2 optimization==




===PCR===
===PCR===
* [[Image:20120110_PCRa.png|800 px]]
* [[Image:20130307_PCR.png|800 px]]




===Gel===
===Gel===


{| {{table}} style="text-align: center"
|-
! Pour !! Load !! Run
|-
| 270 mL 1x TAE + 5.4 g agarose || 2 µL gel load dye + 4 µL PCR product || 160 V
|-
| 27 µL GelRed || 6 µL ladder || 45 minutes
|}


*for SCOC MP1 Batch4 and SCOC MP2 optimization


[[Image:20120110_Gela.png|800 px]]


* mispriming everywhere - primer pair not usable for CNHY
[[Image:20130307_Gel.png|800 px]]
* lots of mispriming, but may be able to use conditions indicated by orange face ([[Image:orangefrownyface.png|20 px]]), if needed, under stringent conditions
 
* favorable conditions indicated by [[Image:greenhappyface.png|25 px]]
* good enough - send




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:: [[Image:20130226_Frag.png|900 px]]
:: [[Image:20130307_Fraga.png|900 px]]
:: [[Image:20130307_Fragb.png|150 px]]




* FedEx Tracking# [http://www.fedex.com/Tracking?language=english&cntry_code=us&tracknumbers=801124151778 801124151778]
* FedEx Tracking# [http://www.fedex.com/Tracking?language=english&cntry_code=us&tracknumbers=801124151745 801124151745]
:: [[Image:20130226_FedEx.png|700 px]]
:: [[Image:20130307_FedEx.png|700 px]]




* UAGC Submission# PPTX
* UAGC Submission# KWWX and YPFS




* FedEx picked up 2:44pm, 26 February
* FedEx picked up 3:01pm, 7 March
* FedEx delivered 9:29am, 27 February
* FedEx delivered 9:10am, 8 March
* UAGC received 10:58pm, 27 February
* UAGC received 11:04am, 8 March
* UAGC completed 10:41am, 28 February
* UAGC completed 10:23am, 11 March




Revision as of 16:14, 12 March 2013

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SCOC MP2 optimization

PCR


Gel

  • for SCOC MP1 Batch4 and SCOC MP2 optimization


  • good enough - send


Fragment Analysis Submission



  • UAGC Submission# KWWX and YPFS


  • FedEx picked up 3:01pm, 7 March
  • FedEx delivered 9:10am, 8 March
  • UAGC received 11:04am, 8 March
  • UAGC completed 10:23am, 11 March




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