User:Tara K. Luckau/Notebook/Team ConGen/2013/03/12: Difference between revisions

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==CNTI Acos5==
==SCOC MP2 optimization==
 
 
===PCR===
* [[Image:20120110_PCRa.png|800 px]]
 
 
===Gel===
 
{| {{table}} style="text-align: center"
|-
! Pour !! Load !! Run
|-
| 270 mL 1x TAE + 5.4 g agarose || 2 µL gel load dye + 4 µL PCR product || 160 V
|-
| 27 µL GelRed || 6 µL ladder || 45 minutes
|}
 
 
[[Image:20120110_Gela.png|800 px]]
 
* mispriming everywhere - primer pair not usable for CNHY
* lots of mispriming, but may be able to use conditions indicated by orange face ([[Image:orangefrownyface.png|20 px]]), if needed, under stringent conditions
* favorable conditions indicated by [[Image:greenhappyface.png|25 px]]




Line 38: Line 15:




:: [[Image:20130226_Frag.png|900 px]]
:: [[Image:20130312_Frag.png|900 px]]




* FedEx Tracking# [http://www.fedex.com/Tracking?language=english&cntry_code=us&tracknumbers=801124151778 801124151778]
* FedEx Tracking# [http://www.fedex.com/Tracking?language=english&cntry_code=us&tracknumbers=801124151723 801124151723]
:: [[Image:20130226_FedEx.png|700 px]]
:: [[Image:20130312_FedEx.png|700 px]]




* UAGC Submission# PPTX
* UAGC Submission# DZAA




* FedEx picked up 2:44pm, 26 February
* FedEx picked up 2:57pm, 12 March
* FedEx delivered 9:29am, 27 February
* FedEx delivered ????am, 13 March
* UAGC received 10:58pm, 27 February
* UAGC received ????am, 13 March
* UAGC completed 10:41am, 28 February
* UAGC completed ????am, 14 March




Revision as of 16:26, 12 March 2013

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SCOC MP2 optimization

Fragment Analysis Submission



  • UAGC Submission# DZAA


  • FedEx picked up 2:57pm, 12 March
  • FedEx delivered ????am, 13 March
  • UAGC received ????am, 13 March
  • UAGC completed ????am, 14 March




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