User:Tara K. Luckau/Notebook/Team ConGen/2013/04/17: Difference between revisions

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* redo it ALL to figure out what the problem is
* redo it ALL to figure out what the problem is
* potential problems:
* potential problems:
** '''DNA - run 4 samples, not just the 2 controls'''
:# '''DNA - run 4 samples, not just the 2 controls'''
** fluorophore - can re-order labelled primers, but only after testing other options because of price
:# fluorophore - can re-order labelled primers, but only after testing other options because of price
** primer interactions - none to be concerned about
:# primer interactions - none to be concerned about
** cycler - avoid using Techne until properly tested
:# cycler - avoid using Techne until properly tested
** 3730 - Crystal reports no problems with other clients' data
:# 3730 - Crystal reports no problems with other clients' data
** '''uniplex/4-plex/8-plex - just re-do these reactions'''
:# '''uniplex/4-plex/8-plex - just re-do these reactions'''
** Taq - can run 2 replicates using 2 different lots of Taq, but don't know what lot I used for the above failures, so not a good test
:# Taq - can run 2 replicates using 2 different lots of Taq, but don't know what lot I used for the above failures, so not a good test
** Buffer - don't know how to test
:# Buffer - don't know how to test
* tackle the bolded potential problems first (#s 1 and 6)




Revision as of 15:43, 9 June 2013

Tara's Lab Notebook <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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SCOC MP2 State of the Multiplex

Summary of Past Results

  • SW614- A4, A6, B1, B12
    • gelled well
    • amplified well in uniplex
    • amplified well in multiplex with all 8 SW markers


  • multiplex with A4 + A6 + B1 + B12
    • gel blank
    • frag fail  ?!
    • maybe due to Techne cycler?


  • re-do of multiplex with A4 + A6 + B1 + B12
    • (didn't run gel)
    • frag failed again!
    • ran on Techne again - thermalcycler poopy?


  • re-re-do of multiplex with A4 + A6 + B1 + B12
    • gel blank
    • frag failed AGAIN!
    • ran on Opticon  :(


Next Steps

  • redo it ALL to figure out what the problem is
  • potential problems:
  1. DNA - run 4 samples, not just the 2 controls
  2. fluorophore - can re-order labelled primers, but only after testing other options because of price
  3. primer interactions - none to be concerned about
  4. cycler - avoid using Techne until properly tested
  5. 3730 - Crystal reports no problems with other clients' data
  6. uniplex/4-plex/8-plex - just re-do these reactions
  7. Taq - can run 2 replicates using 2 different lots of Taq, but don't know what lot I used for the above failures, so not a good test
  8. Buffer - don't know how to test
  • tackle the bolded potential problems first (#s 1 and 6)



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