User:Tara K. Luckau/Notebook/Team ConGen/2013/04/19: Difference between revisions

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==CNTI Acos5==
==SCOC MP2==
* trouble-shooting MP2 failures




===PCR===
===PCR===
* [[Image:20120110_PCRa.png|800 px]]
* [[Image:20130419_PCRa.png|800 px]]




===Gel===
* [[Image:20130419_PCRb.png|800 px]]
 
{| {{table}} style="text-align: center"
|-
! Pour !! Load !! Run
|-
| 270 mL 1x TAE + 5.4 g agarose || 2 µL gel load dye + 4 µL PCR product || 160 V
|-
| 27 µL GelRed || 6 µL ladder || 45 minutes
|}
 
 
[[Image:20120110_Gela.png|800 px]]
 
* mispriming everywhere - primer pair not usable for CNHY
* lots of mispriming, but may be able to use conditions indicated by orange face ([[Image:orangefrownyface.png|20 px]]), if needed, under stringent conditions
* favorable conditions indicated by [[Image:greenhappyface.png|25 px]]
 
 
===Fragment Analysis Submission===
 
 
:: [[Image:20130226_Frag.png|900 px]]
 
 
* FedEx Tracking# [http://www.fedex.com/Tracking?language=english&cntry_code=us&tracknumbers=801124151778 801124151778]
:: [[Image:20130226_FedEx.png|700 px]]
 
 
* UAGC Submission# PPTX
 
 
* FedEx picked up 2:44pm, 26 February
* FedEx delivered 9:29am, 27 February
* UAGC received 10:58pm, 27 February
* UAGC completed 10:41am, 28 February




Revision as of 14:42, 22 April 2013

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SCOC MP2

  • trouble-shooting MP2 failures


PCR





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