User:Tara K. Luckau/Notebook/Team ConGen/2013/05/22: Difference between revisions

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==CNTI Acos5==
==SCOC MP2==




===PCR===
===PCR===
* [[Image:20130110_PCRa.png|800 px]]




===Gel===
* From last two PCRs, looks like not enough template DNA (controls are strong, scorable; some samples had low amplification that seem would be fine with additional template)
 
* SCOC MP2 Plate5 samples plated in triplicate (plus 5 additional samples from various extraction batches), 2µL then 4µL then 6µL. Dessicated overnight as usual.
{| {{table}} style="text-align: center"
:: [[Image:20130522_PCR.png|800 px]]
|-
* Complications:
! Pour !! Load !! Run
** I derped - forgot to actually put the plate in the cycler (left it in the centrifuge) for the duration of the cycling. Noticed when I went to remove the plate from the cycler, only to find the cycler was empty. Plate was sitting in centrifuge for approx. 1.5 hours.
|-
| 270 mL 1x TAE + 5.4 g agarose || 2 µL gel load dye + 4 µL PCR product || 160 V
|-
| 27 µL GelRed || 6 µL ladder || 45 minutes
|}
 
 
[[Image:20130110_Gela.png|800 px]]
 
* mispriming everywhere - primer pair not usable for CNHY
* lots of mispriming, but may be able to use conditions indicated by orange face ([[Image:orangefrownyface.png|20 px]]), if needed, under stringent conditions
* favorable conditions indicated by [[Image:greenhappyface.png|25 px]]




Line 38: Line 25:




:: [[Image:20130227_Frag.png|900 px]]
* UAGC Submission# HBXH
 
:: [[Image:20130522_Frag.png|900 px]]
 
* FedEx Tracking# [http://www.fedex.com/Tracking?language=english&cntry_code=us&tracknumbers=801124151779 801124151779]
:: [[Image:20130227_FedEx.png|700 px]]




* UAGC Submission# ????
* FedEx Tracking# [http://www.fedex.com/Tracking?language=english&cntry_code=us&tracknumbers=801124713122 801124713122]
:: [[Image:20130522_FedEx.png|700 px]]




* FedEx picked up ____pm, 26 February
* FedEx picked up 4:45pm, 22 May
* FedEx delivered ____am, 27 February
* FedEx delivered 9:37am, 23 May
* UAGC received ____am, 27 February
* UAGC received 11:27am, 23 May
* UAGC completed ____am, 28 February
* UAGC completed 10:33am, 24 May




Revision as of 14:33, 9 June 2013

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SCOC MP2

PCR

  • From last two PCRs, looks like not enough template DNA (controls are strong, scorable; some samples had low amplification that seem would be fine with additional template)
  • SCOC MP2 Plate5 samples plated in triplicate (plus 5 additional samples from various extraction batches), 2µL then 4µL then 6µL. Dessicated overnight as usual.
  • Complications:
    • I derped - forgot to actually put the plate in the cycler (left it in the centrifuge) for the duration of the cycling. Noticed when I went to remove the plate from the cycler, only to find the cycler was empty. Plate was sitting in centrifuge for approx. 1.5 hours.


Fragment Analysis Submission

  • UAGC Submission# HBXH



  • FedEx picked up 4:45pm, 22 May
  • FedEx delivered 9:37am, 23 May
  • UAGC received 11:27am, 23 May
  • UAGC completed 10:33am, 24 May




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