User:Tara K. Luckau/Notebook/Team ConGen/2013/05/22

From OpenWetWare
Jump to navigationJump to search
Tara's Lab Notebook <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>


SCOC MP2

PCR

  • From last two PCRs, looks like not enough template DNA (controls are strong, scorable; some samples had low amplification that seem would be fine with additional template)
  • SCOC MP2 Plate5 samples plated in triplicate (plus 5 additional samples from various extraction batches), 2µL then 4µL then 6µL. Dessicated overnight as usual.
  • Complications:
    • I derped - forgot to actually put the plate in the cycler (left it in the centrifuge) for the duration of the cycling. Noticed when I went to remove the plate from the cycler, only to find the cycler was empty. Plate was sitting in centrifuge for approx. 1.5 hours.


Fragment Analysis Submission



  • UAGC Submission# HBXH


  • FedEx picked up ____pm, 26 February
  • FedEx delivered ____am, 27 February
  • UAGC received ____am, 27 February
  • UAGC completed ____am, 28 February