User:Tara K. Luckau/Notebook/Team ConGen/2013/06/06: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
(Autocreate 2013/06/06 Entry for User:Tara_K._Luckau/Notebook/Team_ConGen)
 
No edit summary
Line 9: Line 9:




==CNTI Acos5==
==SCOC MP2 Plates1-4==




Line 15: Line 15:




* comments
* Plates 1-4 (not batches, to differentiate between SCOC MP1 and SCOC MP2)
:: [[Image:20130110_PCRa.png|800 px]]
:: [[Image:20130606_PCRa1.png|800 px]]
:: [[Image:20130606_PCRa2.png|800 px]]




===Gel===
==ASHY MP1/2/3 Opt==


{| {{table}} style="text-align: center"
|-
! Pour !! Load !! Run
|-
| 270 mL 1x TAE + 5.4 g agarose || 2 µL gel load dye + 4 µL PCR product || 160 V
|-
| 27 µL GelRed || 6 µL ladder || 45 minutes
|}


 
===PCR===
:: [[Image:20130110_Gela.png|800 px]]




* mispriming everywhere - primer pair not usable for CNHY
* Re-do from 18Mar2013 (everything failed unexpectedly)
* lots of mispriming, but may be able to use conditions indicated by orange face ([[Image:orangefrownyface.png|20 px]]), if needed, under stringent conditions
:: [[Image:20130606_PCRb.png|800 px]]
* favorable conditions indicated by [[Image:greenhappyface.png|25 px]]




Line 42: Line 33:




* UAGC Submission# ????
* UAGC Submission# BPSH, HBDA, PMGS, GHDY, QSNF
:: [[Image:20130227_Frag.png|900 px]]
:: [[Image:20130606_Fraga.png|900 px]]
:: [[Image:20130606_Fragb.png|900 px]]




* FedEx Tracking# [http://www.fedex.com/Tracking?language=english&cntry_code=us&tracknumbers=801124151779 801124151779]
* FedEx Tracking# [http://www.fedex.com/Tracking?language=english&cntry_code=us&tracknumbers=801124713100 801124713100]
:: [[Image:20130227_FedEx.png|700 px]]
:: [[Image:20130606_FedEx.png|700 px]]




Revision as of 14:49, 9 June 2013

Tara's Lab Notebook <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>


SCOC MP2 Plates1-4

PCR

  • Plates 1-4 (not batches, to differentiate between SCOC MP1 and SCOC MP2)


ASHY MP1/2/3 Opt

PCR

  • Re-do from 18Mar2013 (everything failed unexpectedly)


Fragment Analysis Submission

  • UAGC Submission# BPSH, HBDA, PMGS, GHDY, QSNF


File:20130606 FedEx.png


  • FedEx picked up ____pm, 26 February
  • FedEx delivered ____am, 27 February
  • UAGC received ____am, 27 February
  • UAGC completed ____am, 28 February




Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Tara_K._Luckau/Notebook/Team_ConGen/2013/06/06&oldid=702080"