User:Tara K. Luckau/Notebook/Team ConGen/Entry Base: Difference between revisions
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* pour into caster, set 2 24-tooth combs, cover with cardboard | * pour into caster, set 2 24-tooth combs, cover with cardboard | ||
* let cool in dark (had to do office hours, sat for about 2 hours) | * let cool in dark (had to do office hours, sat for about 2 hours) | ||
===Load Gel=== | |||
* Top: | |||
# B1 | |||
# C1 | |||
# D1 | |||
# E1 | |||
# F1 | |||
# G1 | |||
# H1 | |||
# Ladder | |||
# A2 | |||
# B2 | |||
# C2 | |||
# D2 | |||
# E2 | |||
# F2 | |||
# G2 | |||
# H2 | |||
# A3 | |||
# B3 | |||
# C3 | |||
# D3 | |||
# E3 | |||
# F3 | |||
# G3 | |||
# H3 | |||
* Bottom: | |||
# A4 | |||
# B4 | |||
# C4 | |||
# D4 | |||
# E4 | |||
# F4 | |||
# G4 | |||
# H4 | |||
# A5 | |||
# B5 | |||
# C5 | |||
# D5 | |||
# E5 | |||
# F5 | |||
# G5 | |||
# H5 | |||
# Ladder | |||
# A6 | |||
# B6 | |||
# C6 | |||
# D6 | |||
# E6 | |||
# F6 | |||
# G6 | |||
===Run Gel=== | |||
* use top volt-box | |||
* Power On | |||
*: DC On | |||
*: Voltmeter: push to right | |||
*: Adjust red knob to 90-120V (using red scale of left-side gauge) | |||
* 90V 3p-4:30p | |||
*: 120V 4:30p-4:45p | |||
==25 October 2010 - Ladder Mix== | ==25 October 2010 - Ladder Mix== | ||
===Manufacturer specifications=== | ===Manufacturer specifications=== |
Revision as of 16:35, 25 October 2010
Tara's Lab Notebook | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page |
27 September 2010 - Ordered Primers
11 October 2010 - PCR Thermalcycler TestingPurpose
Results
Purpose
Results
12 October 2010 - PCR Thermalcycler TestingPurpose
Results
18 October 2010 - PCR Thermalcycler TestingPurpose
Results
18 October 2010 - Primer Rehydration, Scun2Purpose
Calculations
18 October 2010 - dNTP MixPurpose
Calculations
Wet Work
19 October 2010 - PCR Scun2 F-R21 October 2010 - Gel Scun2 F-RMake 1x TAE
Cast Gel
Next Try
25 October 2010 - Gel Scun2 F-RCast Gel
Load Gel
Run Gel
25 October 2010 - Ladder MixManufacturer specifications
Other Knowns
Calculations
Make Ladder Mix
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