User:Tara K. Luckau/Notebook/Team ConGen/Entry Base

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==CNHY Acos5==
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==CNTI Acos5==
===PCR===
===PCR===
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* [[Image:20111023_PCRa.png|800 px]]
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* [[Image:20120110_PCRa.png|800 px]]
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[[Image:20111023_Gel.png|800 px]]
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[[Image:20120110_Gela.png|800 px]]
* mispriming everywhere - primer pair not usable for CNHY
* mispriming everywhere - primer pair not usable for CNHY

Revision as of 13:01, 10 January 2012

Tara's Lab Notebook Main project page

Contents


CNTI Acos5

PCR


Gel

Pour Load Run
270 mL 1x TAE + 5.4 g agarose 2 µL gel load dye + 4 µL PCR product 160 V
27 µL GelRed 6 µL ladder 45 minutes


  • mispriming everywhere - primer pair not usable for CNHY
  • lots of mispriming, but may be able to use conditions indicated by orange face (), if needed, under stringent conditions
  • favorable conditions indicated by




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