User:Tara K. Luckau/Notebook/Team ConGen/Entry Base: Difference between revisions
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|style="background-color: # | |style="background-color: #BCED91"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Tara's Lab Notebook</span> | ||
|style="background-color: # | |style="background-color: #9DB68C" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html> </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}} | ||
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== | __TOC__ | ||
* | |||
==CNTI Acos5== | |||
===PCR=== | |||
* [[Image:20120110_PCRa.png|800 px]] | |||
===Gel=== | |||
{| {{table}} style="text-align: center" | |||
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! Pour !! Load !! Run | |||
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| 270 mL 1x TAE + 5.4 g agarose || 2 µL gel load dye + 4 µL PCR product || 160 V | |||
|- | |||
| 27 µL GelRed || 6 µL ladder || 45 minutes | |||
|} | |||
[[Image:20120110_Gela.png|800 px]] | |||
* mispriming everywhere - primer pair not usable for CNHY | |||
* lots of mispriming, but may be able to use conditions indicated by orange face ([[Image:orangefrownyface.png|20 px]]), if needed, under stringent conditions | |||
* favorable conditions indicated by [[Image:greenhappyface.png|25 px]] | |||
===Fragment Analysis Submission=== | |||
:: [[Image:20130226_Frag.png|900 px]] | |||
* FedEx Tracking# [http://www.fedex.com/Tracking?language=english&cntry_code=us&tracknumbers=801124151778 801124151778] | |||
:: [[Image:20130226_FedEx.png|700 px]] | |||
* UAGC Submission# PPTX | |||
* FedEx picked up 2:44pm, 26 February | |||
* FedEx delivered 9:29am, 27 February | |||
* UAGC received 10:58pm, 27 February | |||
* UAGC completed 10:41am, 28 February | |||
Revision as of 16:02, 12 March 2013
Tara's Lab Notebook | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page | |||||||||
CNTI Acos5PCR
Gel
Fragment Analysis Submission
|