User:Tara K. Luckau/Notebook/Team ConGen/Entry Base: Difference between revisions

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__TOC__
__TOC__
==Task==
* here
* here
** in here
# numbered
# again


===Subsection===
 
* here
==CNTI Acos5==
 
 
===PCR===
* [[Image:20120110_PCRa.png|800 px]]
 
 
===Gel===
 
{| {{table}} style="text-align: center"
|-
! Pour !! Load !! Run
|-
| 270 mL 1x TAE + 5.4 g agarose || 2 µL gel load dye + 4 µL PCR product || 160 V
|-
| 27 µL GelRed || 6 µL ladder || 45 minutes
|}
 
 
[[Image:20120110_Gela.png|800 px]]
 
* mispriming everywhere - primer pair not usable for CNHY
* lots of mispriming, but may be able to use conditions indicated by orange face ([[Image:orangefrownyface.png|20 px]]), if needed, under stringent conditions
* favorable conditions indicated by [[Image:greenhappyface.png|25 px]]
 
 
===Fragment Analysis Submission===
 
 
:: [[Image:20130226_Frag.png|900 px]]
 
 
* FedEx Tracking# [http://www.fedex.com/Tracking?language=english&cntry_code=us&tracknumbers=801124151778 801124151778]
:: [[Image:20130226_FedEx.png|700 px]]
 
 
* UAGC Submission# PPTX
 
 
* FedEx picked up 2:44pm, 26 February
* FedEx delivered 9:29am, 27 February
* UAGC received 10:58pm, 27 February
* UAGC completed 10:41am, 28 February
 





Revision as of 16:02, 12 March 2013

Tara's Lab Notebook <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page


CNTI Acos5

PCR


Gel

Pour Load Run
270 mL 1x TAE + 5.4 g agarose 2 µL gel load dye + 4 µL PCR product 160 V
27 µL GelRed 6 µL ladder 45 minutes


  • mispriming everywhere - primer pair not usable for CNHY
  • lots of mispriming, but may be able to use conditions indicated by orange face (), if needed, under stringent conditions
  • favorable conditions indicated by


Fragment Analysis Submission



  • UAGC Submission# PPTX


  • FedEx picked up 2:44pm, 26 February
  • FedEx delivered 9:29am, 27 February
  • UAGC received 10:58pm, 27 February
  • UAGC completed 10:41am, 28 February