User:Tara K. Luckau/Notebook/Team ConGen/Entry Base: Difference between revisions

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===PCR===
===PCR===
* [[Image:20120110_PCRa.png|800 px]]
* [[Image:20130110_PCRa.png|800 px]]




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[[Image:20120110_Gela.png|800 px]]
[[Image:20130110_Gela.png|800 px]]


* mispriming everywhere - primer pair not usable for CNHY
* mispriming everywhere - primer pair not usable for CNHY

Revision as of 14:12, 7 May 2013

Tara's Lab Notebook <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page


CNTI Acos5

PCR


Gel

Pour Load Run
270 mL 1x TAE + 5.4 g agarose 2 µL gel load dye + 4 µL PCR product 160 V
27 µL GelRed 6 µL ladder 45 minutes


File:20130110 Gela.png

  • mispriming everywhere - primer pair not usable for CNHY
  • lots of mispriming, but may be able to use conditions indicated by orange face (), if needed, under stringent conditions
  • favorable conditions indicated by


Fragment Analysis Submission



  • UAGC Submission# PPTX


  • FedEx picked up 2:44pm, 26 February
  • FedEx delivered 9:29am, 27 February
  • UAGC received 10:58pm, 27 February
  • UAGC completed 10:41am, 28 February




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