User:Tara K. Luckau/Notebook/Team ConGen/Entry Base

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==27 September 2010 - Ordered Primers==
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__TOC__
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* Ordered first set of primers
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* Scun2 and Scun22 (highly variable simple motifs)
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** Lance et al (2009) Conservation Genetics Resources, Sceloporus undulatus
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==CNTI Acos5==
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* Forward and reverse as given in publication, also reverse with pigtail (GTTTCTT) at 5' end, also forward with M13F(-21) tag (TGTAAAACGACGGCCAGT)
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* [[Image:Luckau_Primer20100927.jpg|500 px]]
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* Try all four combinations
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===PCR===
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# F - R
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# F - R pigtail
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# F M13 - R
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* comments
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# F M13 - R pigtail
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:: [[Image:20130110_PCRa.png|800 px]]
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* If they don't hinder efforts of change optimization decisions, then may make optimization of multiplexes cheaper!
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==11 October 2010 - PCR Thermalcycler Testing==
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===Gel===
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{| {{table}} style="text-align: center"
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|-
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! Pour !! Load !! Run
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|-
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| 270 mL 1x TAE + 5.4 g agarose || 2 µL gel load dye + 4 µL PCR product || 160 V
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|-
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| 27 µL GelRed || 6 µL ladder || 45 minutes
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|}
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:: [[Image:20130110_Gela.png|800 px]]
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* mispriming everywhere - primer pair not usable for CNHY
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* lots of mispriming, but may be able to use conditions indicated by orange face ([[Image:orangefrownyface.png|20 px]]), if needed, under stringent conditions
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* favorable conditions indicated by [[Image:greenhappyface.png|25 px]]
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===Fragment Analysis Submission===
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* UAGC Submission# ????
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:: [[Image:20130227_Frag.png|900 px]]
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* FedEx Tracking# [http://www.fedex.com/Tracking?language=english&cntry_code=us&tracknumbers=801124151779 801124151779]
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:: [[Image:20130227_FedEx.png|700 px]]
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* FedEx picked up ____pm, 26 February
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* FedEx delivered ____am, 27 February
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* UAGC received ____am, 27 February
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* UAGC completed ____am, 28 February
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Current revision

Tara's Lab Notebook Main project page

Contents


CNTI Acos5

PCR

  • comments
Image:20130110 PCRa.png


Gel

Pour Load Run
270 mL 1x TAE + 5.4 g agarose 2 µL gel load dye + 4 µL PCR product 160 V
27 µL GelRed 6 µL ladder 45 minutes


Image:20130110 Gela.png


  • mispriming everywhere - primer pair not usable for CNHY
  • lots of mispriming, but may be able to use conditions indicated by orange face (), if needed, under stringent conditions
  • favorable conditions indicated by


Fragment Analysis Submission

  • UAGC Submission# ????
Image:20130227 Frag.png


Image:20130227 FedEx.png


  • FedEx picked up ____pm, 26 February
  • FedEx delivered ____am, 27 February
  • UAGC received ____am, 27 February
  • UAGC completed ____am, 28 February




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