User:Tara K. Luckau/Notebook/Team ConGen/Entry Base

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|style="background-color: #BCED91"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Tara's Lab Notebook</span>
|style="background-color: #BCED91"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Tara's Lab Notebook</span>
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<LNCalendar></LNCalendar>
 
|style="background-color: #9DB68C" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #9DB68C" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
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__TOC__
__TOC__
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==Task==
 
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* here
 
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* here
 
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** in here
 
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# numbered
 
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# again
 
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===Subsection===
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* here
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==CNTI Acos5==
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===PCR===
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* comments
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:: [[Image:20130110_PCRa.png|800 px]]
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===Gel===
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{| {{table}} style="text-align: center"
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|-
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! Pour !! Load !! Run
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|-
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| 270 mL 1x TAE + 5.4 g agarose || 2 µL gel load dye + 4 µL PCR product || 160 V
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|-
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| 27 µL GelRed || 6 µL ladder || 45 minutes
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|}
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:: [[Image:20130110_Gela.png|800 px]]
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 +
 
 +
* mispriming everywhere - primer pair not usable for CNHY
 +
* lots of mispriming, but may be able to use conditions indicated by orange face ([[Image:orangefrownyface.png|20 px]]), if needed, under stringent conditions
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* favorable conditions indicated by [[Image:greenhappyface.png|25 px]]
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 +
 
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===Fragment Analysis Submission===
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 +
 
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* UAGC Submission# ????
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:: [[Image:20130227_Frag.png|900 px]]
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* FedEx Tracking# [http://www.fedex.com/Tracking?language=english&cntry_code=us&tracknumbers=801124151779 801124151779]
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:: [[Image:20130227_FedEx.png|700 px]]
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* FedEx picked up ____pm, 26 February
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* FedEx delivered ____am, 27 February
 +
* UAGC received ____am, 27 February
 +
* UAGC completed ____am, 28 February
 +
 

Current revision

Tara's Lab Notebook Main project page

Contents


CNTI Acos5

PCR

  • comments
Image:20130110 PCRa.png


Gel

Pour Load Run
270 mL 1x TAE + 5.4 g agarose 2 µL gel load dye + 4 µL PCR product 160 V
27 µL GelRed 6 µL ladder 45 minutes


Image:20130110 Gela.png


  • mispriming everywhere - primer pair not usable for CNHY
  • lots of mispriming, but may be able to use conditions indicated by orange face (), if needed, under stringent conditions
  • favorable conditions indicated by


Fragment Analysis Submission

  • UAGC Submission# ????
Image:20130227 Frag.png


Image:20130227 FedEx.png


  • FedEx picked up ____pm, 26 February
  • FedEx delivered ____am, 27 February
  • UAGC received ____am, 27 February
  • UAGC completed ____am, 28 February




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